Antigenicity of hepatitis C virus envelope proteins expressed in Chinese hamster ovary cells

M. Inudoh, Hiroshi Nyunoya, T. Tanaka, M. Hijikata, N. Kato, K. Shimotohno

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A putative second envelope glycoprotein (E2) of hepatitis C virus (HCV) was constitutively produced in a Chinese hamster ovary cell line stably transformed with a plasmid expressing E2 protein under the control of an exogenous promoter and a signal sequence. E2 protein that lacked part of the C-terminal hydrophobic region was glycosylated with high-mannose type oligosaccharides and retained in the cells. On the other hand, E2 protein lacking the entire C-terminal hydrophobic region was glycosylated with complex type oligosaccharides (complex form) and excreted into the culture medium. Immunoreactivity of the high-mannose and complex forms of E2 proteins against sera from HCV infected patients were analyzed. We found that the antigenicity of the complex form of E2 protein was greater than that of the high-mannose form of E2 protein. This result indicated that the complex form of the E2 protein is superior for use in diagnosing HCV infection.

Original languageEnglish
Pages (from-to)1590-1596
Number of pages7
JournalVaccine
Volume14
Issue number17-18
DOIs
Publication statusPublished - 1996 Dec
Externally publishedYes

Fingerprint

Viral Envelope Proteins
Hepatitis C virus
Chinese hamsters
Cricetulus
Hepacivirus
Ovary
Mannose
mannose
Proteins
proteins
cells
Oligosaccharides
oligosaccharides
Transformed Cell Line
Virus Diseases
Protein Sorting Signals
signal peptide
blood proteins
Culture Media
Blood Proteins

Keywords

  • antibody
  • envelope protein
  • hepatitis C virus

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Antigenicity of hepatitis C virus envelope proteins expressed in Chinese hamster ovary cells. / Inudoh, M.; Nyunoya, Hiroshi; Tanaka, T.; Hijikata, M.; Kato, N.; Shimotohno, K.

In: Vaccine, Vol. 14, No. 17-18, 12.1996, p. 1590-1596.

Research output: Contribution to journalArticle

Inudoh, M, Nyunoya, H, Tanaka, T, Hijikata, M, Kato, N & Shimotohno, K 1996, 'Antigenicity of hepatitis C virus envelope proteins expressed in Chinese hamster ovary cells', Vaccine, vol. 14, no. 17-18, pp. 1590-1596. https://doi.org/10.1016/S0264-410X(96)00154-5
Inudoh, M. ; Nyunoya, Hiroshi ; Tanaka, T. ; Hijikata, M. ; Kato, N. ; Shimotohno, K. / Antigenicity of hepatitis C virus envelope proteins expressed in Chinese hamster ovary cells. In: Vaccine. 1996 ; Vol. 14, No. 17-18. pp. 1590-1596.
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