Antimicrobial peptides extend lifespan in Drosophila

Gerrit Loch, Ingo Zinke, Tetsushi Mori, Pilar Carrera, Jonas Schroer, Haruko Takeyama, Michael Hoch

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Antimicrobial peptides (AMPs) are important defense molecules of the innate immune system. High levels of AMPs are induced in response to infections to fight pathogens, whereas moderate levels induced by metabolic stress are thought to shape commensal microbial communities at barrier tissues. We expressed single AMPs in adult flies either ubiquitously or in the gut by using the inducible GeneSwitch system to tightly regulate AMP expression. We found that activation of single AMPs, including Drosocin, resulted in a significant extension of Drosophila lifespan. These animals showed reduced activity of immune pathways over lifetime, less intestinal regenerative processes, reduced stress response and a delayed loss of gut barrier integrity. Furthermore, intestinal Drosocin induction protected the animals against infections with the natural Drosophila pathogen Pseudomonas entomophila, whereas a germ-reduced environment prevented the lifespan extending effect of Drosocin. Our study provides new insights into the crosstalk of innate immunity, intestinal homeostasis and ageing.

    Original languageEnglish
    Article numbere0176689
    JournalPLoS One
    Volume12
    Issue number5
    DOIs
    Publication statusPublished - 2017 May 1

    Fingerprint

    antimicrobial peptides
    Drosophila
    Peptides
    Pathogens
    Animals
    digestive system
    Physiological Stress
    pathogens
    Immune system
    Crosstalk
    Pseudomonas
    Infection
    Innate Immunity
    Diptera
    infection
    microbial communities
    Immune System
    stress response
    homeostasis
    animals

    ASJC Scopus subject areas

    • Medicine(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

    Cite this

    Loch, G., Zinke, I., Mori, T., Carrera, P., Schroer, J., Takeyama, H., & Hoch, M. (2017). Antimicrobial peptides extend lifespan in Drosophila. PLoS One, 12(5), [e0176689]. https://doi.org/10.1371/journal.pone.0176689

    Antimicrobial peptides extend lifespan in Drosophila. / Loch, Gerrit; Zinke, Ingo; Mori, Tetsushi; Carrera, Pilar; Schroer, Jonas; Takeyama, Haruko; Hoch, Michael.

    In: PLoS One, Vol. 12, No. 5, e0176689, 01.05.2017.

    Research output: Contribution to journalArticle

    Loch, G, Zinke, I, Mori, T, Carrera, P, Schroer, J, Takeyama, H & Hoch, M 2017, 'Antimicrobial peptides extend lifespan in Drosophila', PLoS One, vol. 12, no. 5, e0176689. https://doi.org/10.1371/journal.pone.0176689
    Loch G, Zinke I, Mori T, Carrera P, Schroer J, Takeyama H et al. Antimicrobial peptides extend lifespan in Drosophila. PLoS One. 2017 May 1;12(5). e0176689. https://doi.org/10.1371/journal.pone.0176689
    Loch, Gerrit ; Zinke, Ingo ; Mori, Tetsushi ; Carrera, Pilar ; Schroer, Jonas ; Takeyama, Haruko ; Hoch, Michael. / Antimicrobial peptides extend lifespan in Drosophila. In: PLoS One. 2017 ; Vol. 12, No. 5.
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