Antioxidant activities of natural 9-CIS and synthetic all-trans β- carotene assessed by human neutrophil chemiluminescence

Qiang Liu, Katsuhiko Suzuki, Shigeyuki Nakaji, Kazuo Sugawara

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Much attention has been focused on the biological activity of geometrical isomers of β-carotene. In the present study, the in vitro antioxidant activities of a natural 9-cis β-carotene purified from Dunaliella bardawil and synthetic all-trans β-carotene were studied using enhanced human neutrophil chemiluminescence upon stimulation with opsonized zymosan. Lucigenin-dependent chemiluminescence was used for largely measuring superoxide anion (O2) production, luminol-dependent chemiluminescence with sodium azide (NAN3) and horseradish peroxidase (HRP) was used for measuring hydrogen peroxide (H2O2) production, and luminol-dependent chemiluminescence without oxidant metabolism modulator was used for determination of myeloperoxidase (MPO)-mediated production. With β-carotene concentration of 1.4 μ M, neutrophils showed a decrease in lucigenin- dependent chemiluminescence to 75% and 78% of controls (p<0.05) by natural 9- cis β-carotene and synthetic all-trans β-carotene, respectively. With β- carotene concentration of 1.86 μ M, neutrophils also showed a decrease in luminol-dependent chemiluminescence in the presence of NaN3 and HRP to 86% and 81% of controls (p<0.05) by natural 9-cis β -carotene and synthetic all- trans β -carotene, respectively. On the other hand, neutrophils did not show a statistically significant difference in any chemiluminescence assay by the same concentration of natural 9-cis β-carotene and synthetic all-trans β - carotene. These results suggest that, up to the concentration of 1.86 μ M, natural 9-cis β -carotene and synthetic all-trans β-carotene exert equal antioxidant activities assessed by enhanced human neutrophil chemiluminescence.

Original languageEnglish
Pages (from-to)5-14
Number of pages10
JournalNutrition Research
Volume20
Issue number1
DOIs
Publication statusPublished - 2000 Jan
Externally publishedYes

Fingerprint

Carotenoids
Luminescence
Neutrophils
Antioxidants
Luminol
Sodium Azide
Horseradish Peroxidase
Zymosan
Oxidants
Superoxides
Hydrogen Peroxide
Peroxidase

Keywords

  • β-carotene
  • β-carotene isomers
  • Antioxidant
  • Chemiluminescence
  • Dunaliella bardawil
  • Human neutrophils

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Antioxidant activities of natural 9-CIS and synthetic all-trans β- carotene assessed by human neutrophil chemiluminescence. / Liu, Qiang; Suzuki, Katsuhiko; Nakaji, Shigeyuki; Sugawara, Kazuo.

In: Nutrition Research, Vol. 20, No. 1, 01.2000, p. 5-14.

Research output: Contribution to journalArticle

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abstract = "Much attention has been focused on the biological activity of geometrical isomers of β-carotene. In the present study, the in vitro antioxidant activities of a natural 9-cis β-carotene purified from Dunaliella bardawil and synthetic all-trans β-carotene were studied using enhanced human neutrophil chemiluminescence upon stimulation with opsonized zymosan. Lucigenin-dependent chemiluminescence was used for largely measuring superoxide anion (O2) production, luminol-dependent chemiluminescence with sodium azide (NAN3) and horseradish peroxidase (HRP) was used for measuring hydrogen peroxide (H2O2) production, and luminol-dependent chemiluminescence without oxidant metabolism modulator was used for determination of myeloperoxidase (MPO)-mediated production. With β-carotene concentration of 1.4 μ M, neutrophils showed a decrease in lucigenin- dependent chemiluminescence to 75{\%} and 78{\%} of controls (p<0.05) by natural 9- cis β-carotene and synthetic all-trans β-carotene, respectively. With β- carotene concentration of 1.86 μ M, neutrophils also showed a decrease in luminol-dependent chemiluminescence in the presence of NaN3 and HRP to 86{\%} and 81{\%} of controls (p<0.05) by natural 9-cis β -carotene and synthetic all- trans β -carotene, respectively. On the other hand, neutrophils did not show a statistically significant difference in any chemiluminescence assay by the same concentration of natural 9-cis β-carotene and synthetic all-trans β - carotene. These results suggest that, up to the concentration of 1.86 μ M, natural 9-cis β -carotene and synthetic all-trans β-carotene exert equal antioxidant activities assessed by enhanced human neutrophil chemiluminescence.",
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