Antioxidant activity of Ge-132, a synthetic organic germanium, on cultured mammalian cells

Takeyoshi Wada, Takashi Hanyu, Kota Nozaki, Kosuke Kataoka, Tomoro Kawatani, Toru Asahi, Naoya Sawamura*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Ge-132 is a synthetic organic germanium that is used as a dietary supplement. The antioxidant activity of Ge-132 on cultured mammalian cells was investigated in this study. First, Ge-132 cytotoxicity on mammalian cultured cells was determined by measuring lactate dehydrogenase (LDH) levels. Ge-132 had no cytotoxic effect on three different cell lines. Second, the cell proliferative effect of Ge-132 was determined by measuring ATP content of whole cells and counting them. Ge-132 treatment of Chinese hamster ovary (CHO-K1) and SH-SY5Y cells promoted cell proliferation in a dose-dependent manner. Finally, antioxidant activity of Ge-132 against hydrogen peroxide-induced oxidative stress was determined by measuring the levels of intracellular reactive oxygen species (ROS) and carbonylated proteins. Pre-incubation of CHO-K1 and SH-SY5Y cells with Ge-132 suppressed intracellular ROS production and carbonylated protein levels induced by hydrogen peroxide. Our results suggest that Ge-132 has antioxidant activity against hydrogen peroxide-induced oxidative stress.

Original languageEnglish
Pages (from-to)749-753
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Issue number5
Publication statusPublished - 2018


  • Ge-132
  • Hydrogen peroxide
  • Organic germanium
  • Oxidative stress

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science


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