Antioxidant activity of Ge-132, a synthetic organic germanium, on cultured mammalian cells

Takeyoshi Wada, Takashi Hanyu, Kota Nozaki, Kosuke Kataoka, Tomoro Kawatani, Toru Asahi, Naoya Sawamura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Ge-132 is a synthetic organic germanium that is used as a dietary supplement. The antioxidant activity of Ge-132 on cultured mammalian cells was investigated in this study. First, Ge-132 cytotoxicity on mammalian cultured cells was determined by measuring lactate dehydrogenase (LDH) levels. Ge-132 had no cytotoxic effect on three different cell lines. Second, the cell proliferative effect of Ge-132 was determined by measuring ATP content of whole cells and counting them. Ge-132 treatment of Chinese hamster ovary (CHO-K1) and SH-SY5Y cells promoted cell proliferation in a dose-dependent manner. Finally, antioxidant activity of Ge-132 against hydrogen peroxide-induced oxidative stress was determined by measuring the levels of intracellular reactive oxygen species (ROS) and carbonylated proteins. Pre-incubation of CHO-K1 and SH-SY5Y cells with Ge-132 suppressed intracellular ROS production and carbonylated protein levels induced by hydrogen peroxide. Our results suggest that Ge-132 has antioxidant activity against hydrogen peroxide-induced oxidative stress.

Original languageEnglish
Pages (from-to)749-753
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume41
Issue number5
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

Germanium
Cultured Cells
Antioxidants
Hydrogen Peroxide
Reactive Oxygen Species
Oxidative Stress
proxigermanium
Dietary Supplements
Cricetulus
L-Lactate Dehydrogenase
Ovary
Proteins
Adenosine Triphosphate
Cell Proliferation
Cell Line

Keywords

  • Ge-132
  • Hydrogen peroxide
  • Organic germanium
  • Oxidative stress

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

Antioxidant activity of Ge-132, a synthetic organic germanium, on cultured mammalian cells. / Wada, Takeyoshi; Hanyu, Takashi; Nozaki, Kota; Kataoka, Kosuke; Kawatani, Tomoro; Asahi, Toru; Sawamura, Naoya.

In: Biological and Pharmaceutical Bulletin, Vol. 41, No. 5, 01.01.2018, p. 749-753.

Research output: Contribution to journalArticle

@article{82bd40f469404d7fabedc253390a7576,
title = "Antioxidant activity of Ge-132, a synthetic organic germanium, on cultured mammalian cells",
abstract = "Ge-132 is a synthetic organic germanium that is used as a dietary supplement. The antioxidant activity of Ge-132 on cultured mammalian cells was investigated in this study. First, Ge-132 cytotoxicity on mammalian cultured cells was determined by measuring lactate dehydrogenase (LDH) levels. Ge-132 had no cytotoxic effect on three different cell lines. Second, the cell proliferative effect of Ge-132 was determined by measuring ATP content of whole cells and counting them. Ge-132 treatment of Chinese hamster ovary (CHO-K1) and SH-SY5Y cells promoted cell proliferation in a dose-dependent manner. Finally, antioxidant activity of Ge-132 against hydrogen peroxide-induced oxidative stress was determined by measuring the levels of intracellular reactive oxygen species (ROS) and carbonylated proteins. Pre-incubation of CHO-K1 and SH-SY5Y cells with Ge-132 suppressed intracellular ROS production and carbonylated protein levels induced by hydrogen peroxide. Our results suggest that Ge-132 has antioxidant activity against hydrogen peroxide-induced oxidative stress.",
keywords = "Ge-132, Hydrogen peroxide, Organic germanium, Oxidative stress",
author = "Takeyoshi Wada and Takashi Hanyu and Kota Nozaki and Kosuke Kataoka and Tomoro Kawatani and Toru Asahi and Naoya Sawamura",
year = "2018",
month = "1",
day = "1",
doi = "10.1248/bpb.b17-00949",
language = "English",
volume = "41",
pages = "749--753",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "5",

}

TY - JOUR

T1 - Antioxidant activity of Ge-132, a synthetic organic germanium, on cultured mammalian cells

AU - Wada, Takeyoshi

AU - Hanyu, Takashi

AU - Nozaki, Kota

AU - Kataoka, Kosuke

AU - Kawatani, Tomoro

AU - Asahi, Toru

AU - Sawamura, Naoya

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Ge-132 is a synthetic organic germanium that is used as a dietary supplement. The antioxidant activity of Ge-132 on cultured mammalian cells was investigated in this study. First, Ge-132 cytotoxicity on mammalian cultured cells was determined by measuring lactate dehydrogenase (LDH) levels. Ge-132 had no cytotoxic effect on three different cell lines. Second, the cell proliferative effect of Ge-132 was determined by measuring ATP content of whole cells and counting them. Ge-132 treatment of Chinese hamster ovary (CHO-K1) and SH-SY5Y cells promoted cell proliferation in a dose-dependent manner. Finally, antioxidant activity of Ge-132 against hydrogen peroxide-induced oxidative stress was determined by measuring the levels of intracellular reactive oxygen species (ROS) and carbonylated proteins. Pre-incubation of CHO-K1 and SH-SY5Y cells with Ge-132 suppressed intracellular ROS production and carbonylated protein levels induced by hydrogen peroxide. Our results suggest that Ge-132 has antioxidant activity against hydrogen peroxide-induced oxidative stress.

AB - Ge-132 is a synthetic organic germanium that is used as a dietary supplement. The antioxidant activity of Ge-132 on cultured mammalian cells was investigated in this study. First, Ge-132 cytotoxicity on mammalian cultured cells was determined by measuring lactate dehydrogenase (LDH) levels. Ge-132 had no cytotoxic effect on three different cell lines. Second, the cell proliferative effect of Ge-132 was determined by measuring ATP content of whole cells and counting them. Ge-132 treatment of Chinese hamster ovary (CHO-K1) and SH-SY5Y cells promoted cell proliferation in a dose-dependent manner. Finally, antioxidant activity of Ge-132 against hydrogen peroxide-induced oxidative stress was determined by measuring the levels of intracellular reactive oxygen species (ROS) and carbonylated proteins. Pre-incubation of CHO-K1 and SH-SY5Y cells with Ge-132 suppressed intracellular ROS production and carbonylated protein levels induced by hydrogen peroxide. Our results suggest that Ge-132 has antioxidant activity against hydrogen peroxide-induced oxidative stress.

KW - Ge-132

KW - Hydrogen peroxide

KW - Organic germanium

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=85046701468&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046701468&partnerID=8YFLogxK

U2 - 10.1248/bpb.b17-00949

DO - 10.1248/bpb.b17-00949

M3 - Article

VL - 41

SP - 749

EP - 753

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 5

ER -