Antiprothrombin autoantibodies in severe preeclampsia and abortion

Takayuki Akimoto, Takao Akama, Masahiro Saitoh, Ichiro Kono, Takayuki Sumida

Research output: Contribution to journalArticle

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Abstract

PURPOSE: We examined the levels of autoantibodies against prethrombin-1 and fragment-1 in pregnant women to determine the type of autoantibodies that were associated with severe preeclampsia or spontaneous abortion. SUBJECTS AND METHODS: We measured autoantibodies bound to prothrombin, prethrombin-1, and fragment-1 by using an enzyme-linked immunosorbent assay (ELISA) in 12 healthy nonpregnant women, 36 women with normal pregnancies, 28 pregnant women with severe preeclampsia, and 19 pregnant women who subsequently had spontaneous abortion. RESULTS: Plasma samples in 10 (36%) of the 28 women with severe preeclampsia and 11 (58%) of the 19 women with spontaneous abortion were positive for antiprothrombin antibodies as compared with 3 (9%) of the 36 women with normal pregancies. All 11 of the positive samples from women who had spontaneous abortions were positive for antiprethrombin-1 antibody, but only 1 was positive for antifragment-1 antibody. The mean (± SD) titer of antiprethrombin-1 antibodies in patients with spontaneous abortion (36 ± 9 U) was higher than that in women with normal pregnancies (10 ± 4 U; P <0.01). Antiprethrombin-1 antibody was detected in only 2 women with severe preeclampsia, whereas all 10 women with antiprethrombin antibodies were positive for antifragment-1 antibody. The antifragment-1 antibody titer in patients with severe preeclampsia (49 ± 15 U) was higher than in women with normal pregnancies (13 ± 6 U, P <0.01). CONCLUSIONS: There is a strong and specific association between various types of antiprothrombin antibodies with severe preeclampsia and spontaneous abortion.

Original languageEnglish
Pages (from-to)188-191
Number of pages4
JournalAmerican Journal of Medicine
Volume110
Issue number3
DOIs
Publication statusPublished - 2001 Feb 15
Externally publishedYes

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Pre-Eclampsia
Autoantibodies
Spontaneous Abortion
Antibodies
Pregnant Women
Pregnancy
Prothrombin
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Nursing(all)

Cite this

Antiprothrombin autoantibodies in severe preeclampsia and abortion. / Akimoto, Takayuki; Akama, Takao; Saitoh, Masahiro; Kono, Ichiro; Sumida, Takayuki.

In: American Journal of Medicine, Vol. 110, No. 3, 15.02.2001, p. 188-191.

Research output: Contribution to journalArticle

Akimoto, Takayuki ; Akama, Takao ; Saitoh, Masahiro ; Kono, Ichiro ; Sumida, Takayuki. / Antiprothrombin autoantibodies in severe preeclampsia and abortion. In: American Journal of Medicine. 2001 ; Vol. 110, No. 3. pp. 188-191.
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abstract = "PURPOSE: We examined the levels of autoantibodies against prethrombin-1 and fragment-1 in pregnant women to determine the type of autoantibodies that were associated with severe preeclampsia or spontaneous abortion. SUBJECTS AND METHODS: We measured autoantibodies bound to prothrombin, prethrombin-1, and fragment-1 by using an enzyme-linked immunosorbent assay (ELISA) in 12 healthy nonpregnant women, 36 women with normal pregnancies, 28 pregnant women with severe preeclampsia, and 19 pregnant women who subsequently had spontaneous abortion. RESULTS: Plasma samples in 10 (36{\%}) of the 28 women with severe preeclampsia and 11 (58{\%}) of the 19 women with spontaneous abortion were positive for antiprothrombin antibodies as compared with 3 (9{\%}) of the 36 women with normal pregancies. All 11 of the positive samples from women who had spontaneous abortions were positive for antiprethrombin-1 antibody, but only 1 was positive for antifragment-1 antibody. The mean (± SD) titer of antiprethrombin-1 antibodies in patients with spontaneous abortion (36 ± 9 U) was higher than that in women with normal pregnancies (10 ± 4 U; P <0.01). Antiprethrombin-1 antibody was detected in only 2 women with severe preeclampsia, whereas all 10 women with antiprethrombin antibodies were positive for antifragment-1 antibody. The antifragment-1 antibody titer in patients with severe preeclampsia (49 ± 15 U) was higher than in women with normal pregnancies (13 ± 6 U, P <0.01). CONCLUSIONS: There is a strong and specific association between various types of antiprothrombin antibodies with severe preeclampsia and spontaneous abortion.",
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AB - PURPOSE: We examined the levels of autoantibodies against prethrombin-1 and fragment-1 in pregnant women to determine the type of autoantibodies that were associated with severe preeclampsia or spontaneous abortion. SUBJECTS AND METHODS: We measured autoantibodies bound to prothrombin, prethrombin-1, and fragment-1 by using an enzyme-linked immunosorbent assay (ELISA) in 12 healthy nonpregnant women, 36 women with normal pregnancies, 28 pregnant women with severe preeclampsia, and 19 pregnant women who subsequently had spontaneous abortion. RESULTS: Plasma samples in 10 (36%) of the 28 women with severe preeclampsia and 11 (58%) of the 19 women with spontaneous abortion were positive for antiprothrombin antibodies as compared with 3 (9%) of the 36 women with normal pregancies. All 11 of the positive samples from women who had spontaneous abortions were positive for antiprethrombin-1 antibody, but only 1 was positive for antifragment-1 antibody. The mean (± SD) titer of antiprethrombin-1 antibodies in patients with spontaneous abortion (36 ± 9 U) was higher than that in women with normal pregnancies (10 ± 4 U; P <0.01). Antiprethrombin-1 antibody was detected in only 2 women with severe preeclampsia, whereas all 10 women with antiprethrombin antibodies were positive for antifragment-1 antibody. The antifragment-1 antibody titer in patients with severe preeclampsia (49 ± 15 U) was higher than in women with normal pregnancies (13 ± 6 U, P <0.01). CONCLUSIONS: There is a strong and specific association between various types of antiprothrombin antibodies with severe preeclampsia and spontaneous abortion.

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