Antisera to calreticulin inhibits sperm motility in mice

Masahisa Nakamura*, Shigeru Oshio, Akihiro Tamura, Shoichi Okinaga, Kiyoshi Arai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Mouse sperm were rapidly immobilized when exposed to rabbit antisera against rat calreticulin. The inhibition of sperm motility was concentration dependent at dilutions 1:50-350. The velocity of sperm did not change significantly as long as they were motile. Neither motility nor velocity of sperm was affected by adding sheep antisera to bovine calmodulin. The antisera to calreticulin also inhibited in vitro fertilization of mouse eggs. Using the avidin-biotin-peroxidase complex method, the antigen was found to be localized in the acrosome of sperm. The results indicate that calreticulin is present in the acrosome of mouse sperm and may play an important role in sperm motility.

Original languageEnglish
Pages (from-to)984-990
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 1992 Jul 31
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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