Antisera to calreticulin inhibits sperm motility in mice

Masahisa Nakamura, Shigeru Oshio, Akihiro Tamura, Shoichi Okinaga, Kiyoshi Arai

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Mouse sperm were rapidly immobilized when exposed to rabbit antisera against rat calreticulin. The inhibition of sperm motility was concentration dependent at dilutions 1:50-350. The velocity of sperm did not change significantly as long as they were motile. Neither motility nor velocity of sperm was affected by adding sheep antisera to bovine calmodulin. The antisera to calreticulin also inhibited in vitro fertilization of mouse eggs. Using the avidin-biotin-peroxidase complex method, the antigen was found to be localized in the acrosome of sperm. The results indicate that calreticulin is present in the acrosome of mouse sperm and may play an important role in sperm motility.

Original languageEnglish
Pages (from-to)984-990
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume186
Issue number2
DOIs
Publication statusPublished - 1992 Jul 31
Externally publishedYes

Fingerprint

Calreticulin
Sperm Motility
Spermatozoa
Immune Sera
Acrosome
Avidin
Calmodulin
Biotin
Peroxidase
Dilution
Rats
Antigens
Fertilization in Vitro
Eggs
Sheep
Rabbits

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Antisera to calreticulin inhibits sperm motility in mice. / Nakamura, Masahisa; Oshio, Shigeru; Tamura, Akihiro; Okinaga, Shoichi; Arai, Kiyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 186, No. 2, 31.07.1992, p. 984-990.

Research output: Contribution to journalArticle

Nakamura, Masahisa ; Oshio, Shigeru ; Tamura, Akihiro ; Okinaga, Shoichi ; Arai, Kiyoshi. / Antisera to calreticulin inhibits sperm motility in mice. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 186, No. 2. pp. 984-990.
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