Antitumor effects of Ninjin-youei-to and its mechanisms

F. Yasutomi, N. Kawate, H. Takahashi, T. Ohira, H. Kato

Research output: Contribution to journalArticle

Abstract

Some Chinese herbal medicines have been reported to have immunomodulating activities, which may have antitumor effects. In the present study, we examined the antitumor effect of Ninjin-youei-to in mice receiving implantation of tumor cells. In C57BL/6 mice, Ninjin-youei-to was mixed with chow and one week after initiation of the experiment, they received intradermal transplantation with 2 X 105 cells of the Lewis lung tumor cell line into the right hind leg. The mean tumor size was significantly (p<0.05) suppressed at 2 and 3 weeks after the transplantation in the group treated with Ninjin-youei-to (n=7) compared with the group treated with solvent alone (n=7). In order to understand the action mechanism of Ninjin-youei-to, blood was collected and the spleen was excised followed by measurement of NK cell activity, Lyt-land Lyt-2. In the group treated with Ninjin-youei-to, NK cell activity, Lyt-land Lyt-2 were significantly (p<0. 05) elevated at 0 and 2 weeks after the transplantation. The antitumor effect of combined treatment of Ninjin-youei-to and cisplatin (CDDP) was also investigated using Lewis lung tumor-bearing mice. The combined use of CDDP with Ninjin-youei-to resulted in significant augmentation of the antitumor activity at 1 (p<0.003) and 2 weeks (p<0.001)after the transplantation compared with CDDP alone. In addition, the combined treatment significantly (p=0.021) prolonged the survival rate compared with CDDP alone. Although there were significant differences in NK cell activity, Lyt-land Lyt-2 (p=0.008, 0.037 and 0.042, respectively) in blood and spleen cells obtained at 1 week after the transplantation, no significant difference was observed thereafter.

Original languageEnglish
Pages (from-to)616-623
Number of pages8
JournalJournal of Tokyo Medical College
Volume55
Issue number5
Publication statusPublished - 1997 Dec 1

Keywords

  • Antitumor effect
  • Immunomodulating activities
  • Ninjin-youei-to

ASJC Scopus subject areas

  • Medicine(all)

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    Yasutomi, F., Kawate, N., Takahashi, H., Ohira, T., & Kato, H. (1997). Antitumor effects of Ninjin-youei-to and its mechanisms. Journal of Tokyo Medical College, 55(5), 616-623.