Abstract
The viability of rat uterine natural killer (NK) cells in vitro was examined. Uterine NK cells were obtained from rats on days 10 and 16 of pregnancy and they were cultured in RPMI 1640 medium supplemented with 10% v/v fetal calf serum in the presence or absence of human recombinant IL-2 or cycloheximide for 24, 48, 72 and 96 h. In both preparations of cells collected on days 10 and 16 of pregnancy, about 60% of the cells died during the first 48 h of culture regardless of the presence of IL-2. Gel electrophoresis of genomic DNA showed ladder fragmentation with 180 to 200-bp fragments, indicating that the cell death was due to apoptosis. Apoptosis of uterine NK cells in vitro was promoted, not prevented, by cycloheximide. The remaining uterine NK cells (40%) were maintained alive under these culture conditions for, at least, 96 h. Therefore, rat uterine NK cells undergo apoptosis and are consist of subpopulations with referring viabilities in vitro.
| Original language | English |
|---|---|
| Pages (from-to) | 219-224 |
| Number of pages | 6 |
| Journal | Journal of Reproduction and Development |
| Volume | 42 |
| Issue number | 4 |
| Publication status | Published - 1996 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Cycloheximide
- Uterine NK cell
ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
Apoptosis of rat uterine NK cells in vitro. / Fukasawa, Norihiro; Hattori, Naka; Zehavi-Willner, Tova; Kiso, Yasuo; Shiota, Kunio; Ogawa, Tomoya.
In: Journal of Reproduction and Development, Vol. 42, No. 4, 1996, p. 219-224.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apoptosis of rat uterine NK cells in vitro
AU - Fukasawa, Norihiro
AU - Hattori, Naka
AU - Zehavi-Willner, Tova
AU - Kiso, Yasuo
AU - Shiota, Kunio
AU - Ogawa, Tomoya
PY - 1996
Y1 - 1996
N2 - The viability of rat uterine natural killer (NK) cells in vitro was examined. Uterine NK cells were obtained from rats on days 10 and 16 of pregnancy and they were cultured in RPMI 1640 medium supplemented with 10% v/v fetal calf serum in the presence or absence of human recombinant IL-2 or cycloheximide for 24, 48, 72 and 96 h. In both preparations of cells collected on days 10 and 16 of pregnancy, about 60% of the cells died during the first 48 h of culture regardless of the presence of IL-2. Gel electrophoresis of genomic DNA showed ladder fragmentation with 180 to 200-bp fragments, indicating that the cell death was due to apoptosis. Apoptosis of uterine NK cells in vitro was promoted, not prevented, by cycloheximide. The remaining uterine NK cells (40%) were maintained alive under these culture conditions for, at least, 96 h. Therefore, rat uterine NK cells undergo apoptosis and are consist of subpopulations with referring viabilities in vitro.
AB - The viability of rat uterine natural killer (NK) cells in vitro was examined. Uterine NK cells were obtained from rats on days 10 and 16 of pregnancy and they were cultured in RPMI 1640 medium supplemented with 10% v/v fetal calf serum in the presence or absence of human recombinant IL-2 or cycloheximide for 24, 48, 72 and 96 h. In both preparations of cells collected on days 10 and 16 of pregnancy, about 60% of the cells died during the first 48 h of culture regardless of the presence of IL-2. Gel electrophoresis of genomic DNA showed ladder fragmentation with 180 to 200-bp fragments, indicating that the cell death was due to apoptosis. Apoptosis of uterine NK cells in vitro was promoted, not prevented, by cycloheximide. The remaining uterine NK cells (40%) were maintained alive under these culture conditions for, at least, 96 h. Therefore, rat uterine NK cells undergo apoptosis and are consist of subpopulations with referring viabilities in vitro.
KW - Apoptosis
KW - Cycloheximide
KW - Uterine NK cell
UR - http://www.scopus.com/inward/record.url?scp=0346281856&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0346281856&partnerID=8YFLogxK
M3 - Article
VL - 42
SP - 219
EP - 224
JO - Journal of Reproduction and Development
JF - Journal of Reproduction and Development
SN - 0916-8818
IS - 4
ER -