Apparent diffusion time of oxygen from blood to tissue in rat cerebral cortex: Implication for tissue oxygen dynamics during brain functions

Kazuto Masamoto, Jeff Kershaw, Masakatsu Ureshi, Naosada Takizawa, Hirosuke Kobayashi, Kazuo Tanishita, Iwao Kanno

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To investigate the dynamics of tissue oxygen demand and supply during brain functions, we simultaneously recorded PO2 and local cerebral blood flow (LCBF) with an oxygen microelectrode and laser Doppler flowmetry, respectively, in rat somatosensory cortex. Electrical hind-limb stimuli were applied for 1, 2, and 5 s to vary the duration of evoked cerebral metabolic rate of oxygen (CMRO2). The electrical stimulation induced a robust increase in PO2 (4-9 Torr at peak) after an increase in LCBF (14-26% at peak). A consistent lag of ∼1.2 s (0.6-2.3 s for individual animals) in the PO2 relative to LCBF was found, irrespective of stimulus length. It is argued that the lag in PO2 was predominantly caused by the time required for oxygen to diffuse through tissue. During brain functions, the supply of fresh oxygen further lagged because of the latency of LCBF onset (∼0.4 s). The results indicate that the tissue oxygen supports excess demand until the arrival of fresh oxygen. However, a large drop in PO2 was not observed, indicating that the evoked neural activity demands little extra oxygen or that the time course of excess demand is as slow as the increase in supply. Thus the dynamics of PO2 during brain functions predominantly depend on the time course of LCBF. Possible factors influencing the lag between demand and supply are discussed, including vascular spacing, reactivity of the vessels, and diffusivity of oxygen.

Original languageEnglish
Pages (from-to)1352-1358
Number of pages7
JournalJournal of Applied Physiology
Volume103
Issue number4
DOIs
Publication statusPublished - 2007 Oct
Externally publishedYes

Fingerprint

Cerebral Cortex
Cerebrovascular Circulation
Oxygen
Brain
Laser-Doppler Flowmetry
Somatosensory Cortex
Microelectrodes
Electric Stimulation
Blood Vessels
Extremities

Keywords

  • Brain tissue oxygen tension
  • Cerebral blood flow
  • Functional brain imaging
  • Oxygen transport

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Apparent diffusion time of oxygen from blood to tissue in rat cerebral cortex : Implication for tissue oxygen dynamics during brain functions. / Masamoto, Kazuto; Kershaw, Jeff; Ureshi, Masakatsu; Takizawa, Naosada; Kobayashi, Hirosuke; Tanishita, Kazuo; Kanno, Iwao.

In: Journal of Applied Physiology, Vol. 103, No. 4, 10.2007, p. 1352-1358.

Research output: Contribution to journalArticle

Masamoto, Kazuto ; Kershaw, Jeff ; Ureshi, Masakatsu ; Takizawa, Naosada ; Kobayashi, Hirosuke ; Tanishita, Kazuo ; Kanno, Iwao. / Apparent diffusion time of oxygen from blood to tissue in rat cerebral cortex : Implication for tissue oxygen dynamics during brain functions. In: Journal of Applied Physiology. 2007 ; Vol. 103, No. 4. pp. 1352-1358.
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