Applicability of chemiluminescence to assess the degree of operative stress in patients undergoing spinal surgery

Kenji Kowatari, Katsuhiko Suzuki, Satoru Kudo, Mutsuo Yamada, Qiang Liu, Kazuma Danjo, Takashi Umeda, Shigeyuki Nakaji, Kazuo Sugawara

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study examined the applicability of luminol-dependent chemiluminescence (CL) response of neutrophils to assess the degree of stress of spinal surgery by measuring the capacity of circulating neutrophils to produce reactive oxygen species and the levels of serum cytokines: interleukin(IL)-1β, IL-6, IL-8, tumour necrosis factor (TNF)-? and granulocyte colony stimulating factor (G-CSF). Ten male patients underwent spinal surgery. Peripheral blood samples, collected before and after the operation and the next morning, were used for measuring the CL response of neutrophils stimulated with opsonized zymosan and measuring the levels of serum cytokines. The operative stress induced leukocytosis, particularly granulocytosis, and increased serum IL-6 and G-CSF significantly. However, there was no significant change in the luminol-dependent CL response of neutrophils or the levels of serum IL-1β. IL-8 and TNF-α throughout the experimental period. These results suggest that, at least in the early postoperative period, operative stress does not prime the circulating neutrophils, and thus the CL response of neutrophils is not appropriate to assess the degree of stress of spinal surgery.

Original languageEnglish
Pages (from-to)331-334
Number of pages4
JournalLuminescence
Volume14
Issue number6
Publication statusPublished - 1999 Nov
Externally publishedYes

Fingerprint

Chemiluminescence
Luminescence
Surgery
Neutrophils
Luminol
Granulocyte Colony-Stimulating Factor
Interleukin-8
Interleukin-1
Interleukin-6
Tumor Necrosis Factor-alpha
Cytokines
Serum
Zymosan
Reactive Oxygen Species
Blood
Leukocytosis
Postoperative Period

Keywords

  • Chemiluminescence
  • Cytokine
  • Neutrophil
  • Operation
  • SIRS

ASJC Scopus subject areas

  • Biophysics

Cite this

Kowatari, K., Suzuki, K., Kudo, S., Yamada, M., Liu, Q., Danjo, K., ... Sugawara, K. (1999). Applicability of chemiluminescence to assess the degree of operative stress in patients undergoing spinal surgery. Luminescence, 14(6), 331-334.

Applicability of chemiluminescence to assess the degree of operative stress in patients undergoing spinal surgery. / Kowatari, Kenji; Suzuki, Katsuhiko; Kudo, Satoru; Yamada, Mutsuo; Liu, Qiang; Danjo, Kazuma; Umeda, Takashi; Nakaji, Shigeyuki; Sugawara, Kazuo.

In: Luminescence, Vol. 14, No. 6, 11.1999, p. 331-334.

Research output: Contribution to journalArticle

Kowatari, K, Suzuki, K, Kudo, S, Yamada, M, Liu, Q, Danjo, K, Umeda, T, Nakaji, S & Sugawara, K 1999, 'Applicability of chemiluminescence to assess the degree of operative stress in patients undergoing spinal surgery', Luminescence, vol. 14, no. 6, pp. 331-334.
Kowatari, Kenji ; Suzuki, Katsuhiko ; Kudo, Satoru ; Yamada, Mutsuo ; Liu, Qiang ; Danjo, Kazuma ; Umeda, Takashi ; Nakaji, Shigeyuki ; Sugawara, Kazuo. / Applicability of chemiluminescence to assess the degree of operative stress in patients undergoing spinal surgery. In: Luminescence. 1999 ; Vol. 14, No. 6. pp. 331-334.
@article{b5ad8384723849fe86c44fd9356d29cd,
title = "Applicability of chemiluminescence to assess the degree of operative stress in patients undergoing spinal surgery",
abstract = "This study examined the applicability of luminol-dependent chemiluminescence (CL) response of neutrophils to assess the degree of stress of spinal surgery by measuring the capacity of circulating neutrophils to produce reactive oxygen species and the levels of serum cytokines: interleukin(IL)-1β, IL-6, IL-8, tumour necrosis factor (TNF)-? and granulocyte colony stimulating factor (G-CSF). Ten male patients underwent spinal surgery. Peripheral blood samples, collected before and after the operation and the next morning, were used for measuring the CL response of neutrophils stimulated with opsonized zymosan and measuring the levels of serum cytokines. The operative stress induced leukocytosis, particularly granulocytosis, and increased serum IL-6 and G-CSF significantly. However, there was no significant change in the luminol-dependent CL response of neutrophils or the levels of serum IL-1β. IL-8 and TNF-α throughout the experimental period. These results suggest that, at least in the early postoperative period, operative stress does not prime the circulating neutrophils, and thus the CL response of neutrophils is not appropriate to assess the degree of stress of spinal surgery.",
keywords = "Chemiluminescence, Cytokine, Neutrophil, Operation, SIRS",
author = "Kenji Kowatari and Katsuhiko Suzuki and Satoru Kudo and Mutsuo Yamada and Qiang Liu and Kazuma Danjo and Takashi Umeda and Shigeyuki Nakaji and Kazuo Sugawara",
year = "1999",
month = "11",
language = "English",
volume = "14",
pages = "331--334",
journal = "Luminescence",
issn = "1522-7235",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

TY - JOUR

T1 - Applicability of chemiluminescence to assess the degree of operative stress in patients undergoing spinal surgery

AU - Kowatari, Kenji

AU - Suzuki, Katsuhiko

AU - Kudo, Satoru

AU - Yamada, Mutsuo

AU - Liu, Qiang

AU - Danjo, Kazuma

AU - Umeda, Takashi

AU - Nakaji, Shigeyuki

AU - Sugawara, Kazuo

PY - 1999/11

Y1 - 1999/11

N2 - This study examined the applicability of luminol-dependent chemiluminescence (CL) response of neutrophils to assess the degree of stress of spinal surgery by measuring the capacity of circulating neutrophils to produce reactive oxygen species and the levels of serum cytokines: interleukin(IL)-1β, IL-6, IL-8, tumour necrosis factor (TNF)-? and granulocyte colony stimulating factor (G-CSF). Ten male patients underwent spinal surgery. Peripheral blood samples, collected before and after the operation and the next morning, were used for measuring the CL response of neutrophils stimulated with opsonized zymosan and measuring the levels of serum cytokines. The operative stress induced leukocytosis, particularly granulocytosis, and increased serum IL-6 and G-CSF significantly. However, there was no significant change in the luminol-dependent CL response of neutrophils or the levels of serum IL-1β. IL-8 and TNF-α throughout the experimental period. These results suggest that, at least in the early postoperative period, operative stress does not prime the circulating neutrophils, and thus the CL response of neutrophils is not appropriate to assess the degree of stress of spinal surgery.

AB - This study examined the applicability of luminol-dependent chemiluminescence (CL) response of neutrophils to assess the degree of stress of spinal surgery by measuring the capacity of circulating neutrophils to produce reactive oxygen species and the levels of serum cytokines: interleukin(IL)-1β, IL-6, IL-8, tumour necrosis factor (TNF)-? and granulocyte colony stimulating factor (G-CSF). Ten male patients underwent spinal surgery. Peripheral blood samples, collected before and after the operation and the next morning, were used for measuring the CL response of neutrophils stimulated with opsonized zymosan and measuring the levels of serum cytokines. The operative stress induced leukocytosis, particularly granulocytosis, and increased serum IL-6 and G-CSF significantly. However, there was no significant change in the luminol-dependent CL response of neutrophils or the levels of serum IL-1β. IL-8 and TNF-α throughout the experimental period. These results suggest that, at least in the early postoperative period, operative stress does not prime the circulating neutrophils, and thus the CL response of neutrophils is not appropriate to assess the degree of stress of spinal surgery.

KW - Chemiluminescence

KW - Cytokine

KW - Neutrophil

KW - Operation

KW - SIRS

UR - http://www.scopus.com/inward/record.url?scp=0033218252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033218252&partnerID=8YFLogxK

M3 - Article

C2 - 10602303

AN - SCOPUS:0033218252

VL - 14

SP - 331

EP - 334

JO - Luminescence

JF - Luminescence

SN - 1522-7235

IS - 6

ER -