Applicability of chemiluminescence to assess the degree of operative stress in patients undergoing spinal surgery

Kenji Kowatari, Katsuhiko Suzuki, Satoru Kudo, Mutsuo Yamada, Qiang Liu, Kazuma Danjo, Takashi Umeda, Shigeyuki Nakaji, Kazuo Sugawara

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study examined the applicability of luminol-dependent chemiluminescence (CL) response of neutrophils to assess the degree of stress of spinal surgery by measuring the capacity of circulating neutrophils to produce reactive oxygen species and the levels of serum cytokines: interleukin(IL)-1β, IL-6, IL-8, tumour necrosis factor (TNF)-? and granulocyte colony stimulating factor (G-CSF). Ten male patients underwent spinal surgery. Peripheral blood samples, collected before and after the operation and the next morning, were used for measuring the CL response of neutrophils stimulated with opsonized zymosan and measuring the levels of serum cytokines. The operative stress induced leukocytosis, particularly granulocytosis, and increased serum IL-6 and G-CSF significantly. However, there was no significant change in the luminol-dependent CL response of neutrophils or the levels of serum IL-1β. IL-8 and TNF-α throughout the experimental period. These results suggest that, at least in the early postoperative period, operative stress does not prime the circulating neutrophils, and thus the CL response of neutrophils is not appropriate to assess the degree of stress of spinal surgery.

Original languageEnglish
Pages (from-to)331-334
Number of pages4
JournalLuminescence
Volume14
Issue number6
DOIs
Publication statusPublished - 1999

Keywords

  • Chemiluminescence
  • Cytokine
  • Neutrophil
  • Operation
  • SIRS

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)

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