Application of bacterial magnetic particles as novel DNA carriers for ballistic transformation of a marine cyanobacterium

Haruko Takeyama, Akira Yamazawa, Chikashi Nakamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Bacterial magnetite particles (BMPs) of 50 to 100nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gene expression were confirmed by Southern hybridization and CAT assay, respectively. The BMPs were also observed in the cyanobacterial cells by transmission electron microscopy. These results suggested that BMPs can be used as carriers for introducing DNA into bacterial cells.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalBiotechnology Techniques
Volume9
Issue number5
DOIs
Publication statusPublished - 1995 May
Externally publishedYes

Fingerprint

Ferrosoferric Oxide
Cyanobacteria
Magnetite
Ballistics
DNA
Synechococcus
Bacterial DNA
Firearms
Transmission Electron Microscopy
Gene expression
Assays
Transmission electron microscopy
Gene Expression

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

Application of bacterial magnetic particles as novel DNA carriers for ballistic transformation of a marine cyanobacterium. / Takeyama, Haruko; Yamazawa, Akira; Nakamura, Chikashi; Matsunaga, Tadashi.

In: Biotechnology Techniques, Vol. 9, No. 5, 05.1995, p. 355-360.

Research output: Contribution to journalArticle

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