Application of bacterial magnetic particles as novel DNA carriers for ballistic transformation of a marine cyanobacterium

Haruko Takeyama; Akira Yamazawa; Chikashi Nakamura; Tadashi Matsunaga

doi:10.1007/BF00638870

Application of bacterial magnetic particles as novel DNA carriers for ballistic transformation of a marine cyanobacterium

Haruko Takeyama^*, Akira Yamazawa, Chikashi Nakamura, Tadashi Matsunaga

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Bacterial magnetite particles (BMPs) of 50 to 100nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gene expression were confirmed by Southern hybridization and CAT assay, respectively. The BMPs were also observed in the cyanobacterial cells by transmission electron microscopy. These results suggested that BMPs can be used as carriers for introducing DNA into bacterial cells.

Original language	English
Pages (from-to)	355-360
Number of pages	6
Journal	Biotechnology Techniques
Volume	9
Issue number	5
DOIs	https://doi.org/10.1007/BF00638870
Publication status	Published - 1995 May 1
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Applied Microbiology and Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF00638870

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abstract = "Bacterial magnetite particles (BMPs) of 50 to 100nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gene expression were confirmed by Southern hybridization and CAT assay, respectively. The BMPs were also observed in the cyanobacterial cells by transmission electron microscopy. These results suggested that BMPs can be used as carriers for introducing DNA into bacterial cells.",

author = "Haruko Takeyama and Akira Yamazawa and Chikashi Nakamura and Tadashi Matsunaga",

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AU - Takeyama, Haruko

AU - Yamazawa, Akira

AU - Nakamura, Chikashi

AU - Matsunaga, Tadashi

PY - 1995/5/1

Y1 - 1995/5/1

N2 - Bacterial magnetite particles (BMPs) of 50 to 100nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gene expression were confirmed by Southern hybridization and CAT assay, respectively. The BMPs were also observed in the cyanobacterial cells by transmission electron microscopy. These results suggested that BMPs can be used as carriers for introducing DNA into bacterial cells.

AB - Bacterial magnetite particles (BMPs) of 50 to 100nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gene expression were confirmed by Southern hybridization and CAT assay, respectively. The BMPs were also observed in the cyanobacterial cells by transmission electron microscopy. These results suggested that BMPs can be used as carriers for introducing DNA into bacterial cells.

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Application of bacterial magnetic particles as novel DNA carriers for ballistic transformation of a marine cyanobacterium

Abstract

ASJC Scopus subject areas

UN SDGs

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