Application of lifeact reveals F-actin dynamics in arabidopsis thaliana and the liverwort, marchantia polymorpha

Atsuko Era, Motoki Tominaga, Kazuo Ebine, Chie Awai, Chieko Saito, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi, Akihiko Nakano, Takashi Ueda

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Actin plays fundamental roles in a wide array of plant functions, including cell division, cytoplasmic streaming, cell morphogenesis and organelle motility. Imaging the actin cytoskeleton in living cells is a powerful methodology for studying these important phenomena. Several useful probes for live imaging of filamentous actin (F-actin) have been developed, but new versatile probes are still needed. Here, we report the application of a new probe called Lifeact for visualizing F-actin in plant cells. Lifeact is a short peptide comprising 17 amino acids that was derived from yeast Abp140p. We used a LifeactVenus fusion protein for staining F-actin in Arabidopsis thaliana and were able to observe dynamic rearrangements of the actin meshwork in root hair cells. We also used LifeactVenus to visualize the actin cytoskeleton in the liverwort Marchantia polymorpha; this revealed unique and dynamic F-actin motility in liverwort cells. Our results suggest that Lifeact could be a useful tool for studying the actin cytoskeleton in a wide range of plant lineages.

Original languageEnglish
Pages (from-to)1041-1048
Number of pages8
JournalPlant and Cell Physiology
Volume50
Issue number6
DOIs
Publication statusPublished - 2009 Jun
Externally publishedYes

Fingerprint

Marchantia
Hepatophyta
Marchantia polymorpha
Arabidopsis
actin
mosses and liverworts
Actins
Arabidopsis thaliana
microfilaments
Actin Cytoskeleton
cells
Cytoplasmic Streaming
image analysis
cytoplasmic streaming
Plant Cells
root hairs
Morphogenesis
Organelles
Cell Division
morphogenesis

Keywords

  • Actin
  • Arabidopsis thaliana
  • Lifeact
  • Liverwort
  • Marchantia polymorpha

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Application of lifeact reveals F-actin dynamics in arabidopsis thaliana and the liverwort, marchantia polymorpha. / Era, Atsuko; Tominaga, Motoki; Ebine, Kazuo; Awai, Chie; Saito, Chieko; Ishizaki, Kimitsune; Yamato, Katsuyuki T.; Kohchi, Takayuki; Nakano, Akihiko; Ueda, Takashi.

In: Plant and Cell Physiology, Vol. 50, No. 6, 06.2009, p. 1041-1048.

Research output: Contribution to journalArticle

Era, A, Tominaga, M, Ebine, K, Awai, C, Saito, C, Ishizaki, K, Yamato, KT, Kohchi, T, Nakano, A & Ueda, T 2009, 'Application of lifeact reveals F-actin dynamics in arabidopsis thaliana and the liverwort, marchantia polymorpha', Plant and Cell Physiology, vol. 50, no. 6, pp. 1041-1048. https://doi.org/10.1093/pcp/pcp055
Era, Atsuko ; Tominaga, Motoki ; Ebine, Kazuo ; Awai, Chie ; Saito, Chieko ; Ishizaki, Kimitsune ; Yamato, Katsuyuki T. ; Kohchi, Takayuki ; Nakano, Akihiko ; Ueda, Takashi. / Application of lifeact reveals F-actin dynamics in arabidopsis thaliana and the liverwort, marchantia polymorpha. In: Plant and Cell Physiology. 2009 ; Vol. 50, No. 6. pp. 1041-1048.
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