Application of organosilane monolayer template to quantitative evaluation of cancer cell adhesive ability

Takashi Tanii, Kosuke Sasaki, Kota Ichisawa, Takanori Demura, Yuichi Beppu, Hoan Anh Vu, Hoan Thanh Chi, Hideaki Yamamoto, Yuko Sato

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The adhesive ability of two human pancreatic cancer cell lines was evaluated using organosilane monolayer templates (OMTs). Using the OMT, the spreading area of adhered cells can be limited, and this enables us to focus on the initial attachment process of adhesion. Moreover, it becomes possible to arrange the cells in an array and to quantitatively evaluate the number of attached cells. The adhesive ability of the cancer cells cultured on the OMT was controlled by adding (-)-epigallocatechin-3-gallate (EGCG), which blocks a receptor that mediates cell adhesion and is overexpressed in cancer cells. Measurement of the relative ability of the cancer cells to attach to the OMT revealed that the ability for attachment decreased with increasing EGCG concentration. The results agreed well with the western blot analysis, indicating that the OMT can potentially be employed to evaluate the adhesive ability of various cancer cells.

Original languageEnglish
Article number06GL01
JournalJapanese journal of applied physics
Volume50
Issue number6 PART 2
DOIs
Publication statusPublished - 2011 Jun 1

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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    Tanii, T., Sasaki, K., Ichisawa, K., Demura, T., Beppu, Y., Vu, H. A., Chi, H. T., Yamamoto, H., & Sato, Y. (2011). Application of organosilane monolayer template to quantitative evaluation of cancer cell adhesive ability. Japanese journal of applied physics, 50(6 PART 2), [06GL01]. https://doi.org/10.1143/JJAP.50.06GL01