Application of organosilane monolayer template to quantitative evaluation of cancer cell adhesive ability

Takashi Tanii, Kosuke Sasaki, Kota Ichisawa, Takanori Demura, Yuichi Beppu, Hoan Anh Vu, Hoan Thanh Chi, Hideaki Yamamoto, Yuko Sato

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    The adhesive ability of two human pancreatic cancer cell lines was evaluated using organosilane monolayer templates (OMTs). Using the OMT, the spreading area of adhered cells can be limited, and this enables us to focus on the initial attachment process of adhesion. Moreover, it becomes possible to arrange the cells in an array and to quantitatively evaluate the number of attached cells. The adhesive ability of the cancer cells cultured on the OMT was controlled by adding (-)-epigallocatechin-3-gallate (EGCG), which blocks a receptor that mediates cell adhesion and is overexpressed in cancer cells. Measurement of the relative ability of the cancer cells to attach to the OMT revealed that the ability for attachment decreased with increasing EGCG concentration. The results agreed well with the western blot analysis, indicating that the OMT can potentially be employed to evaluate the adhesive ability of various cancer cells.

    Original languageEnglish
    Article number06GL01
    JournalJapanese Journal of Applied Physics
    Volume50
    Issue number6 PART 2
    DOIs
    Publication statusPublished - 2011 Jun

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    adhesives
    Monolayers
    Adhesives
    templates
    cancer
    Cells
    evaluation
    gallates
    cells
    cultured cells
    attachment
    adhesion
    Cell adhesion
    Adhesion

    ASJC Scopus subject areas

    • Engineering(all)
    • Physics and Astronomy(all)

    Cite this

    Application of organosilane monolayer template to quantitative evaluation of cancer cell adhesive ability. / Tanii, Takashi; Sasaki, Kosuke; Ichisawa, Kota; Demura, Takanori; Beppu, Yuichi; Vu, Hoan Anh; Chi, Hoan Thanh; Yamamoto, Hideaki; Sato, Yuko.

    In: Japanese Journal of Applied Physics, Vol. 50, No. 6 PART 2, 06GL01, 06.2011.

    Research output: Contribution to journalArticle

    Tanii, T, Sasaki, K, Ichisawa, K, Demura, T, Beppu, Y, Vu, HA, Chi, HT, Yamamoto, H & Sato, Y 2011, 'Application of organosilane monolayer template to quantitative evaluation of cancer cell adhesive ability', Japanese Journal of Applied Physics, vol. 50, no. 6 PART 2, 06GL01. https://doi.org/10.1143/JJAP.50.06GL01
    Tanii, Takashi ; Sasaki, Kosuke ; Ichisawa, Kota ; Demura, Takanori ; Beppu, Yuichi ; Vu, Hoan Anh ; Chi, Hoan Thanh ; Yamamoto, Hideaki ; Sato, Yuko. / Application of organosilane monolayer template to quantitative evaluation of cancer cell adhesive ability. In: Japanese Journal of Applied Physics. 2011 ; Vol. 50, No. 6 PART 2.
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