TY - GEN
T1 - Application of thermal lens microscopy and sweeping for highly sensitive detection in electrophoretic analysis on cycloolefin polymer microchips
AU - Kitagawa, F.
AU - Tsuneka, T.
AU - Akimoto, Y.
AU - Mizuno, J.
AU - Shoji, S.
AU - Otsuka, K.
PY - 2005/1/1
Y1 - 2005/1/1
N2 - This paper reports an application of cycloolefin polymer (ZEONEX) as a new commercially available plastic material for microchip electrophoresis (MCE), and an improvement of the detectability for serum and chiral analyses by using sweeping and a thermal lens microscope (TLM) as a detection scheme. In the serum analysis, proteins were successfully detected as sharp peaks on the ZEONEX microchips, while on bare quartz microchips only a broader and weaker peak was observed. On the other hand, chiral analysis of 1-aminoindan on the ZEONEX chip under the sweeping condition provided an efficient separation with a 52-fold sensitivity enhancement.
AB - This paper reports an application of cycloolefin polymer (ZEONEX) as a new commercially available plastic material for microchip electrophoresis (MCE), and an improvement of the detectability for serum and chiral analyses by using sweeping and a thermal lens microscope (TLM) as a detection scheme. In the serum analysis, proteins were successfully detected as sharp peaks on the ZEONEX microchips, while on bare quartz microchips only a broader and weaker peak was observed. On the other hand, chiral analysis of 1-aminoindan on the ZEONEX chip under the sweeping condition provided an efficient separation with a 52-fold sensitivity enhancement.
KW - Cycloolefin polymer microchip
KW - Microchip electrophoresis
KW - Sweeping
KW - Thermal lens microscope
UR - http://www.scopus.com/inward/record.url?scp=84902489066&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902489066&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84902489066
SN - 0974361119
SN - 9780974361116
T3 - Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 331
EP - 333
BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
PB - Transducer Research Foundation
T2 - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Y2 - 9 October 2005 through 13 October 2005
ER -