Application of thermal lens microscopy and sweeping for highly sensitive detection in electrophoretic analysis on cycloolefin polymer microchips

F. Kitagawa, T. Tsuneka, Y. Akimoto, J. Mizuno, S. Shoji, K. Otsuka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports an application of cycloolefin polymer (ZEONEX) as a new commercially available plastic material for microchip electrophoresis (MCE), and an improvement of the detectability for serum and chiral analyses by using sweeping and a thermal lens microscope (TLM) as a detection scheme. In the serum analysis, proteins were successfully detected as sharp peaks on the ZEONEX microchips, while on bare quartz microchips only a broader and weaker peak was observed. On the other hand, chiral analysis of 1-aminoindan on the ZEONEX chip under the sweeping condition provided an efficient separation with a 52-fold sensitivity enhancement.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages331-333
Number of pages3
ISBN (Print)0974361119, 9780974361116
Publication statusPublished - 2005 Jan 1
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: 2005 Oct 92005 Oct 13

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Conference

Conference9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period05/10/905/10/13

Keywords

  • Cycloolefin polymer microchip
  • Microchip electrophoresis
  • Sweeping
  • Thermal lens microscope

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Kitagawa, F., Tsuneka, T., Akimoto, Y., Mizuno, J., Shoji, S., & Otsuka, K. (2005). Application of thermal lens microscopy and sweeping for highly sensitive detection in electrophoretic analysis on cycloolefin polymer microchips. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 331-333). (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1). Transducer Research Foundation.