Application of thermal lens microscopy and sweeping for highly sensitive detection in electrophoretic analysis on cycloolefin polymer microchips

F. Kitagawa, T. Tsuneka, Y. Akimoto, Jun Mizuno, Shuichi Shoji, K. Otsuka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports an application of cycloolefin polymer (ZEONEX) as a new commercially available plastic material for microchip electrophoresis (MCE), and an improvement of the detectability for serum and chiral analyses by using sweeping and a thermal lens microscope (TLM) as a detection scheme. In the serum analysis, proteins were successfully detected as sharp peaks on the ZEONEX microchips, while on bare quartz microchips only a broader and weaker peak was observed. On the other hand, chiral analysis of 1-aminoindan on the ZEONEX chip under the sweeping condition provided an efficient separation with a 52-fold sensitivity enhancement.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages331-333
Number of pages3
Volume1
ISBN (Print)0974361119, 9780974361116
Publication statusPublished - 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA
Duration: 2005 Oct 92005 Oct 13

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CityBoston, MA
Period05/10/905/10/13

Fingerprint

Quartz
Electrophoresis
Blood Proteins
Lenses
Microscopic examination
Polymers
Microscopes
Plastics
Proteins
Hot Temperature
1-aminoindan

Keywords

  • Cycloolefin polymer microchip
  • Microchip electrophoresis
  • Sweeping
  • Thermal lens microscope

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Kitagawa, F., Tsuneka, T., Akimoto, Y., Mizuno, J., Shoji, S., & Otsuka, K. (2005). Application of thermal lens microscopy and sweeping for highly sensitive detection in electrophoretic analysis on cycloolefin polymer microchips. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (Vol. 1, pp. 331-333). Transducer Research Foundation.

Application of thermal lens microscopy and sweeping for highly sensitive detection in electrophoretic analysis on cycloolefin polymer microchips. / Kitagawa, F.; Tsuneka, T.; Akimoto, Y.; Mizuno, Jun; Shoji, Shuichi; Otsuka, K.

Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. p. 331-333.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kitagawa, F, Tsuneka, T, Akimoto, Y, Mizuno, J, Shoji, S & Otsuka, K 2005, Application of thermal lens microscopy and sweeping for highly sensitive detection in electrophoretic analysis on cycloolefin polymer microchips. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. vol. 1, Transducer Research Foundation, pp. 331-333, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, 05/10/9.
Kitagawa F, Tsuneka T, Akimoto Y, Mizuno J, Shoji S, Otsuka K. Application of thermal lens microscopy and sweeping for highly sensitive detection in electrophoretic analysis on cycloolefin polymer microchips. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1. Transducer Research Foundation. 2005. p. 331-333
Kitagawa, F. ; Tsuneka, T. ; Akimoto, Y. ; Mizuno, Jun ; Shoji, Shuichi ; Otsuka, K. / Application of thermal lens microscopy and sweeping for highly sensitive detection in electrophoretic analysis on cycloolefin polymer microchips. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. pp. 331-333
@inproceedings{a57b2582c49e467d95d1dd6cac49fdb5,
title = "Application of thermal lens microscopy and sweeping for highly sensitive detection in electrophoretic analysis on cycloolefin polymer microchips",
abstract = "This paper reports an application of cycloolefin polymer (ZEONEX) as a new commercially available plastic material for microchip electrophoresis (MCE), and an improvement of the detectability for serum and chiral analyses by using sweeping and a thermal lens microscope (TLM) as a detection scheme. In the serum analysis, proteins were successfully detected as sharp peaks on the ZEONEX microchips, while on bare quartz microchips only a broader and weaker peak was observed. On the other hand, chiral analysis of 1-aminoindan on the ZEONEX chip under the sweeping condition provided an efficient separation with a 52-fold sensitivity enhancement.",
keywords = "Cycloolefin polymer microchip, Microchip electrophoresis, Sweeping, Thermal lens microscope",
author = "F. Kitagawa and T. Tsuneka and Y. Akimoto and Jun Mizuno and Shuichi Shoji and K. Otsuka",
year = "2005",
language = "English",
isbn = "0974361119",
volume = "1",
pages = "331--333",
booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Transducer Research Foundation",

}

TY - GEN

T1 - Application of thermal lens microscopy and sweeping for highly sensitive detection in electrophoretic analysis on cycloolefin polymer microchips

AU - Kitagawa, F.

AU - Tsuneka, T.

AU - Akimoto, Y.

AU - Mizuno, Jun

AU - Shoji, Shuichi

AU - Otsuka, K.

PY - 2005

Y1 - 2005

N2 - This paper reports an application of cycloolefin polymer (ZEONEX) as a new commercially available plastic material for microchip electrophoresis (MCE), and an improvement of the detectability for serum and chiral analyses by using sweeping and a thermal lens microscope (TLM) as a detection scheme. In the serum analysis, proteins were successfully detected as sharp peaks on the ZEONEX microchips, while on bare quartz microchips only a broader and weaker peak was observed. On the other hand, chiral analysis of 1-aminoindan on the ZEONEX chip under the sweeping condition provided an efficient separation with a 52-fold sensitivity enhancement.

AB - This paper reports an application of cycloolefin polymer (ZEONEX) as a new commercially available plastic material for microchip electrophoresis (MCE), and an improvement of the detectability for serum and chiral analyses by using sweeping and a thermal lens microscope (TLM) as a detection scheme. In the serum analysis, proteins were successfully detected as sharp peaks on the ZEONEX microchips, while on bare quartz microchips only a broader and weaker peak was observed. On the other hand, chiral analysis of 1-aminoindan on the ZEONEX chip under the sweeping condition provided an efficient separation with a 52-fold sensitivity enhancement.

KW - Cycloolefin polymer microchip

KW - Microchip electrophoresis

KW - Sweeping

KW - Thermal lens microscope

UR - http://www.scopus.com/inward/record.url?scp=84902489066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902489066&partnerID=8YFLogxK

M3 - Conference contribution

SN - 0974361119

SN - 9780974361116

VL - 1

SP - 331

EP - 333

BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Transducer Research Foundation

ER -