Aptamer-based biosensor for sensitive PDGF detection using diamond transistor

A. Rahim Ruslinda, Shinya Tajima, Yoko Ishii, Yuichiro Ishiyama, Robert Edgington, Hiroshi Kawarada

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    The detection of platelet-derived growth factor (PDGF) via a solution-gate field-effect transistor (SGFET) has been demonstrated for the first time using aptamers immobilized on a diamond surface. Upon introduction of PDGF to the immobilized aptamer, a shift of 31.7. mV in the negative direction is observed at a source-drain current of -50 μA. A shift of 32.3. mV in the positive direction is detected after regeneration by SDS solution, indicating that the static measurement returns to its original value. These SGFETs operate stably within the large potential window of diamond (>3.0. V), and hence the surface channel does not need passivating with a thick insulating layer. Thereof, the immobilized aptamer channels have been exposed directly to the electrolyte solution without a gate insulator. Immobilization is achieved via aptamers covalently bonding to amine sites, thereby increasing the sensitivity of the biosensors. Diamond SGFETs have potential for the detection of PDGF and show durability against biological degradation after repeated usage and regeneration.

    Original languageEnglish
    Pages (from-to)1599-1604
    Number of pages6
    JournalBiosensors and Bioelectronics
    Volume26
    Issue number4
    DOIs
    Publication statusPublished - 2010 Dec 15

    Fingerprint

    Diamond
    Platelet-Derived Growth Factor
    Biosensing Techniques
    Platelets
    Biosensors
    Diamonds
    Transistors
    Regeneration
    Gates (transistor)
    Drain current
    Immobilization
    Electrolytes
    Amines
    Durability
    Degradation
    Intercellular Signaling Peptides and Proteins
    Direction compound

    Keywords

    • Aptamer
    • Biosensor
    • Diamond transistor
    • Platelet-derived growth factor

    ASJC Scopus subject areas

    • Biophysics
    • Biomedical Engineering
    • Biotechnology
    • Electrochemistry

    Cite this

    Aptamer-based biosensor for sensitive PDGF detection using diamond transistor. / Ruslinda, A. Rahim; Tajima, Shinya; Ishii, Yoko; Ishiyama, Yuichiro; Edgington, Robert; Kawarada, Hiroshi.

    In: Biosensors and Bioelectronics, Vol. 26, No. 4, 15.12.2010, p. 1599-1604.

    Research output: Contribution to journalArticle

    Ruslinda, A. Rahim ; Tajima, Shinya ; Ishii, Yoko ; Ishiyama, Yuichiro ; Edgington, Robert ; Kawarada, Hiroshi. / Aptamer-based biosensor for sensitive PDGF detection using diamond transistor. In: Biosensors and Bioelectronics. 2010 ; Vol. 26, No. 4. pp. 1599-1604.
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    AU - Edgington, Robert

    AU - Kawarada, Hiroshi

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