Abstract
The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM.
| Original language | English |
|---|---|
| Article number | 27001 |
| Journal | Applied Physics Express |
| Volume | 4 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2011 Feb |
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ASJC Scopus subject areas
- Engineering(all)
- Physics and Astronomy(all)
Cite this
Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method. / Ishii, Yoko; Tajima, Shinya; Kawarada, Hiroshi.
In: Applied Physics Express, Vol. 4, No. 2, 27001, 02.2011.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method
AU - Ishii, Yoko
AU - Tajima, Shinya
AU - Kawarada, Hiroshi
PY - 2011/2
Y1 - 2011/2
N2 - The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM.
AB - The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM.
UR - http://www.scopus.com/inward/record.url?scp=79951617181&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79951617181&partnerID=8YFLogxK
U2 - 10.1143/APEX.4.027001
DO - 10.1143/APEX.4.027001
M3 - Article
AN - SCOPUS:79951617181
VL - 4
JO - Applied Physics Express
JF - Applied Physics Express
SN - 1882-0778
IS - 2
M1 - 27001
ER -