Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method

Yoko Ishii; Shinya Tajima; Hiroshi Kawarada

doi:10.1143/APEX.4.027001

Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method

Yoko Ishii, Shinya Tajima, Hiroshi Kawarada

School of Fundamental Science and Engineering

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM.

Original language	English
Article number	27001
Journal	Applied Physics Express
Volume	4
Issue number	2
DOIs	https://doi.org/10.1143/APEX.4.027001
Publication status	Published - 2011 Feb 1

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy(all)

Access to Document

10.1143/APEX.4.027001

Fingerprint Dive into the research topics of 'Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method'. Together they form a unique fingerprint.

Cite this

@article{d10e0debb92441c3926fe4f6cc0ca89c,

title = "Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method",

abstract = "The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM.",

author = "Yoko Ishii and Shinya Tajima and Hiroshi Kawarada",

year = "2011",

month = feb,

day = "1",

doi = "10.1143/APEX.4.027001",

language = "English",

volume = "4",

journal = "Applied Physics Express",

issn = "1882-0778",

publisher = "Japan Society of Applied Physics",

number = "2",

}

TY - JOUR

T1 - Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method

AU - Ishii, Yoko

AU - Tajima, Shinya

AU - Kawarada, Hiroshi

PY - 2011/2/1

Y1 - 2011/2/1

N2 - The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM.

AB - The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM.

UR - http://www.scopus.com/inward/record.url?scp=79951617181&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79951617181&partnerID=8YFLogxK

U2 - 10.1143/APEX.4.027001

DO - 10.1143/APEX.4.027001

M3 - Article

AN - SCOPUS:79951617181

VL - 4

JO - Applied Physics Express

JF - Applied Physics Express

SN - 1882-0778

IS - 2

M1 - 27001

ER -