Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method

Yoko Ishii; Shinya Tajima; Hiroshi Kawarada

doi:10.1143/APEX.4.027001

Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method

Yoko Ishii, Shinya Tajima, Hiroshi Kawarada

School of Fundamental Science and Engineering

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM.

Original language	English
Article number	27001
Journal	Applied Physics Express
Volume	4
Issue number	2
DOIs	https://doi.org/10.1143/APEX.4.027001
Publication status	Published - 2011 Feb

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy(all)

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abstract = "The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM.",

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Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method

Abstract

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