Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method

Yoko Ishii, Shinya Tajima, Hiroshi Kawarada

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM.

    Original languageEnglish
    Article number27001
    JournalApplied Physics Express
    Volume4
    Issue number2
    DOIs
    Publication statusPublished - 2011 Feb

    Fingerprint

    Platelets
    platelets
    Diamonds
    diamonds
    optics
    stems
    affinity
    Labels
    Dyes
    selectivity
    dyes
    Fluorescence
    fluorescence
    Intercellular Signaling Peptides and Proteins
    sensitivity
    sensors
    Sensors

    ASJC Scopus subject areas

    • Engineering(all)
    • Physics and Astronomy(all)

    Cite this

    Aptasensor for oncoprotein platelet-derived growth factor detection on functionalized diamond surface by signal-off optical method. / Ishii, Yoko; Tajima, Shinya; Kawarada, Hiroshi.

    In: Applied Physics Express, Vol. 4, No. 2, 27001, 02.2011.

    Research output: Contribution to journalArticle

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