Area-specific cell stimulation via surface-mediated gene transfer using apatite-based composite layers

Yushin Yazaki, Ayako Oyane, Yu Sogo, Atsuo Ito, Atsushi Yamazaki, Hideo Tsurushima

    Research output: Contribution to journalArticle

    Abstract

    Surface-mediated gene transfer systems using biocompatible calcium phosphate (CaP)-based composite layers have attracted attention as a tool for controlling cell behaviors. In the present study we aimed to demonstrate the potential of CaP-based composite layers to mediate area-specific dual gene transfer and to stimulate cells on an area-by-area basis in the same well. For this purpose we prepared two pairs of DNA–fibronectin–apatite composite (DF-Ap) layers using a pair of reporter genes and pair of differentiation factor genes. The results of the area-specific dual gene transfer successfully demonstrated that the cells cultured on a pair of DF-Ap layers that were adjacently placed in the same well showed specific gene expression patterns depending on the gene that was immobilized in theunderlying layer. Moreover, preliminary real-time PCR results indicated that multipotential C3H10T1/2 cells may have a potential to change into different types of cells depending on the differentiation factor gene that was immobilized in the underlying layer, even in the same well. Because DF-Ap layers have a potential to mediate area-specific cell stimulation on their surfaces, they could be useful in tissue engineering applications.

    Original languageEnglish
    Pages (from-to)8294-8309
    Number of pages16
    JournalInternational Journal of Molecular Sciences
    Volume16
    Issue number4
    DOIs
    Publication statusPublished - 2015 Apr 14

    Fingerprint

    Gene transfer
    Apatites
    Apatite
    apatites
    stimulation
    genes
    Genes
    composite materials
    Composite materials
    cells
    Calcium phosphate
    calcium phosphates
    Tissue engineering
    Gene expression
    Tissue Engineering
    Reporter Genes
    tissue engineering
    gene expression
    Real-Time Polymerase Chain Reaction
    Cultured Cells

    Keywords

    • Calcium phosphate
    • Dual gene transfer
    • Hydroxyapatite
    • Reverse transfection
    • Scaffold

    ASJC Scopus subject areas

    • Physical and Theoretical Chemistry
    • Organic Chemistry
    • Spectroscopy
    • Inorganic Chemistry
    • Catalysis
    • Molecular Biology
    • Computer Science Applications

    Cite this

    Area-specific cell stimulation via surface-mediated gene transfer using apatite-based composite layers. / Yazaki, Yushin; Oyane, Ayako; Sogo, Yu; Ito, Atsuo; Yamazaki, Atsushi; Tsurushima, Hideo.

    In: International Journal of Molecular Sciences, Vol. 16, No. 4, 14.04.2015, p. 8294-8309.

    Research output: Contribution to journalArticle

    Yazaki, Yushin ; Oyane, Ayako ; Sogo, Yu ; Ito, Atsuo ; Yamazaki, Atsushi ; Tsurushima, Hideo. / Area-specific cell stimulation via surface-mediated gene transfer using apatite-based composite layers. In: International Journal of Molecular Sciences. 2015 ; Vol. 16, No. 4. pp. 8294-8309.
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