Assembly Domain-Based Optogenetic System for the Efficient Control of Cellular Signaling

Akihiro Furuya, Fuun Kawano, Takahiro Nakajima, Yoshibumi Ueda, Moritoshi Sato

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We previously developed the Magnet system, which consists of two distinct Vivid protein variants, one positively and one negatively charged, designated the positive Magnet (pMag) and negative Magnet (nMag), respectively. These two proteins bind to each other through electrostatic interactions, preventing unwanted homodimerization and providing selective light-induced heterodimerization. The Magnet system enables the manipulation of cellular functions such as protein-protein interactions and genome editing, although the system could be improved further. To enhance the ability of pMagFast2 (a pMag variant with fast kinetics) to bind nMag, we introduced several pMagFast2 modules in tandem into a single construct, pMagFast2(3×). However, the expression level of this construct decreased drastically with increasing number of pMagFast2 molecules integrated into a single construct. In the present study, we applied a new approach to improve the Magnet system based on an assembly domain (AD). Among several ADs, the Ca2+/calmodulin-dependent protein kinase IIα association domain (CAD) most enhanced the Magnet system. The present CAD-Magnet system overcame a trade-off issue between the expression level and binding affinity. The CAD-converged 12 pMag photoswitches exhibited a stronger interaction with nMag after blue light irradiation compared with monomeric pMag. Additionally, the CAD played a key role in converging effector proteins as well in a single complex. Owing to these substantial improvements, the CAD-Magnet system combined with Tiam1 allowed us to robustly induce localized formation of vertical ruffles on the apical plasma membrane. The CAD-Magnet system combined with 4D imaging was instrumental in revealing the dynamics of ruffle formation.

Original languageEnglish
Pages (from-to)1086-1095
Number of pages10
JournalACS Synthetic Biology
Volume6
Issue number6
DOIs
Publication statusPublished - 2017 Jun 16
Externally publishedYes

Fingerprint

Optogenetics
Cell signaling
Magnets
Computer aided design
Proteins
Calmodulin
Light
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Cell membranes

Keywords

  • assembly domain
  • Ca/calmodulin-dependent protein kinase IIα (CaMKIIα) association domain (CAD)
  • dorsal ruffle
  • Magnet system
  • optogenetics
  • Tiam1

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Assembly Domain-Based Optogenetic System for the Efficient Control of Cellular Signaling. / Furuya, Akihiro; Kawano, Fuun; Nakajima, Takahiro; Ueda, Yoshibumi; Sato, Moritoshi.

In: ACS Synthetic Biology, Vol. 6, No. 6, 16.06.2017, p. 1086-1095.

Research output: Contribution to journalArticle

Furuya, Akihiro ; Kawano, Fuun ; Nakajima, Takahiro ; Ueda, Yoshibumi ; Sato, Moritoshi. / Assembly Domain-Based Optogenetic System for the Efficient Control of Cellular Signaling. In: ACS Synthetic Biology. 2017 ; Vol. 6, No. 6. pp. 1086-1095.
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