Assignment of imino proton signals of G-C base pairs and magnesium ion binding: An NMR study of bovine mitochondrial tRNA(Ser)(GCU) lacking the entire D arm

Ikuko Hayashi, Takashi Yokogawa, Gota Kawai, Takuya Ueda, Kazuya Nishikawa, Kimitsuna Watanabe

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The mammalian mitochondrial tRNA(Ser)(GCU) (mt tRNA(Ser)(GCU)) has a unique structure in that it lacks the whole D arm. To elucidate its higher-order structure, we synthesized unmodified bovine mt tRNA(Ser)(GCU) using T7 RNA polymerase and measured its 1H-NMR spectrum in the imino proton region. Although the imino proton signals heavily overlapped, we succeeded in assigning all the seven imino proton signals of the G-C base pairs by a combination of base replacement and 15N-labeling of the G residues of a whole tRNA molecule or of the 3'-half fragment. The results indicate that the tRNA possesses the secondary structure that has been supposed on the basis of biochemical studies. Analysis of the effect of the magnesium concentration on the G-C pairs suggests that the acceptor and T stems do not form a co-axial helix, and that the core region of the tRNA does not interact with magnesium ions. These features are significantly different from those of canonical tRNAs. Despite this, it is very likely that the tRNA as a whole takes a nearly L-shape tertiary structure.

Original languageEnglish
Pages (from-to)1115-1122
Number of pages8
JournalJournal of biochemistry
Volume121
Issue number6
DOIs
Publication statusPublished - 1997 Jun

Keywords

  • Assignment of imino proton signals
  • H-NMR
  • Magnesium effect
  • Mitochondrial tRNA
  • N-labeling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Assignment of imino proton signals of G-C base pairs and magnesium ion binding: An NMR study of bovine mitochondrial tRNA(Ser)(GCU) lacking the entire D arm'. Together they form a unique fingerprint.

  • Cite this