Astaxanthin formation in the marine photosynthetic bacterium Rhodovulum sulfidophilum expressing crtI, crtY, crtW and crtZ

Daikichi Mukoyama, Haruko Takeyama, Yutaka Kondo, Tadashi Matsunaga

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

This study reports the production of astaxanthin in the photosynthetic bacterium Rhodovulum sulfidophilum, which has adequate precursor pools and storage capabilities for heterologous carotenoid production. Chemical mutagenesis was carried out using ethylmethane sulfonate to produce mutants with a modified carotenoid biosynthesis pathway downstream of phytoene. Stable green- and gray-colored mutants were selected. Green mutants contained neurosporene or chloroxanthin as their major carotenoid (>90%), while the gray mutants accumulated phytoene. We previously demonstrated the production of β-carotene in Rhodovulum sulfidophilum by cloning the Erythrobacter longus crtI (phytoene dehydrogenase) and crtY (lycopene cyclase) genes. In the present study, an expression vector for astaxanthin production was constructed that contained the Paracoccus crtW (β-carotene oxygenase) and crtZ (β-carotene hydroxylase) genes in addition to the E. longus crtI and crtY genes. A transconjugant, which can synthesize astaxanthin, was successfully generated (2.0 μg g-1 DCW).

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalFEMS Microbiology Letters
Volume265
Issue number1
DOIs
Publication statusPublished - 2006 Dec
Externally publishedYes

Keywords

  • Astaxanthin
  • Carotenoids
  • Marine photosynthetic bacterium
  • Metabolic engineering
  • Rhodovulum sulfidophilum

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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