TY - JOUR
T1 - Astrocytic endfeet re-cover blood vessels after removal by laser ablation
AU - Kubotera, Hideaki
AU - Ikeshima-Kataoka, Hiroko
AU - Hatashita, Yoshiki
AU - Allegra Mascaro, Anna Letizia
AU - Pavone, Francesco Saverio
AU - Inoue, Takafumi
N1 - Funding Information:
The authors gratefully thank Prof. M. Götz, Dr. L. Dimou, Prof. J. Priller, Dr. F. Fernandez, Prof. Y. Ikegaya, Dr. H. Hirase, Dr. T. Mishima, Prof. T. Nemoto and Dr. R. Kawakami for technical advices and suggestions on in vivo imaging with 2PLSM. This work was supported by Grant-in-Aid for Scientific Research of Japan Society for the Promotion of Science (JSPS, HI-K: 15K15532; TI: 23113515 and 23300121), JSPS Core-to-Core Program, A. Advanced Research Networks (HI-K and TI) and Waseda University Grants for Special Research Projects (HI-K: 2012A-872, 2013A-6287, 2013B-127, 2014B-247, 2014K-6137, 2016B-158 and 2016K-196; TI: 2013B-168 and 2017B-238). The work was also supported by the European Union’s Horizon 2020 research and innovation program under grant agreement No. 654148 (Laserlab-Europe).
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - The astrocyte, one of the glial cells, plays many functional roles. These include provision of nutrients from blood vessels to neurons, supply of neurotransmitters and support of blood–brain barrier (BBB) integrity. Astrocytes are known to support the integrity of BBB through maintenance of the tight junction between endothelial cells of blood vessels. However, evidence of its direct contribution to BBB is lacking owing to technical limitations. In this study, astrocytic endfeet covering blood vessels were removed by the laser ablation method with two photon laser scanning microscopy in in vivo mouse brain, and the re-covering of blood vessels with the astrocytic endfeet was observed in about half of the cases. Blood vessels kept their integrity without astrocytic endfoot covers: leakage of plasma marker dyes, Evans Blue or dextran-conjugated fluorescein, was not observed from stripped blood vessels, while ablation of vascular walls induced extravasation of Evans Blue. These results suggest that the astrocytic endfeet covering blood vessels do not contribute to the immediate BBB barrier.
AB - The astrocyte, one of the glial cells, plays many functional roles. These include provision of nutrients from blood vessels to neurons, supply of neurotransmitters and support of blood–brain barrier (BBB) integrity. Astrocytes are known to support the integrity of BBB through maintenance of the tight junction between endothelial cells of blood vessels. However, evidence of its direct contribution to BBB is lacking owing to technical limitations. In this study, astrocytic endfeet covering blood vessels were removed by the laser ablation method with two photon laser scanning microscopy in in vivo mouse brain, and the re-covering of blood vessels with the astrocytic endfeet was observed in about half of the cases. Blood vessels kept their integrity without astrocytic endfoot covers: leakage of plasma marker dyes, Evans Blue or dextran-conjugated fluorescein, was not observed from stripped blood vessels, while ablation of vascular walls induced extravasation of Evans Blue. These results suggest that the astrocytic endfeet covering blood vessels do not contribute to the immediate BBB barrier.
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U2 - 10.1038/s41598-018-37419-4
DO - 10.1038/s41598-018-37419-4
M3 - Article
C2 - 30718555
AN - SCOPUS:85061061921
SN - 2045-2322
VL - 9
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 1263
ER -