Atelocollagen-mediated synthetic small interfering RNA delivery for effective gene silencing in vitro and in vivo.

Yoshiko Minakuchi, Fumitaka Takeshita, Nobuyoshi Kosaka, Hideo Sasaki, Yusuke Yamamoto, Makiko Kouno, Kimi Honma, Shunji Nagahara, Koji Hanai, Akihiko Sano, Takashi Kato, Masaaki Terada, Takahiro Ochiya

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292 Citations (Scopus)

Abstract

Silencing gene expression by siRNAs is rapidly becoming a powerful tool for the genetic analysis of mammalian cells. However, the rapid degradation of siRNA and the limited duration of its action call for an efficient delivery technology. Accordingly, we describe here that Atelocollagen complexed with siRNA is resistant to nucleases and is efficiently transduced into cells, thereby allowing long-term gene silencing. Site-specific in vivo administration of an anti-luciferase siRNA/Atelocollagen complex reduced luciferase expression in a xenografted tumor. Furthermore, Atelocollagen-mediated transfer of siRNA in vivo showed efficient inhibition of tumor growth in an orthotopic xenograft model of a human non-seminomatous germ cell tumor. Thus, for clinical applications of siRNA, an Atelocollagen-based non-viral delivery method could be a reliable approach to achieve maximal function of siRNA in vivo.

Original languageEnglish
Pages (from-to)e109
JournalNucleic acids research
Volume32
Issue number13
DOIs
Publication statusPublished - 2004
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Minakuchi, Y., Takeshita, F., Kosaka, N., Sasaki, H., Yamamoto, Y., Kouno, M., Honma, K., Nagahara, S., Hanai, K., Sano, A., Kato, T., Terada, M., & Ochiya, T. (2004). Atelocollagen-mediated synthetic small interfering RNA delivery for effective gene silencing in vitro and in vivo. Nucleic acids research, 32(13), e109. https://doi.org/10.1093/nar/gnh093