Atg101, a novel mammalian autophagy protein interacting with Atg13

Nao Hosokawa, Takahiro Sasaki, Shun Ichiro Iemura, Tohru Natsume, Taichi Hara, Noboru Mizushima

Research output: Contribution to journalArticle

243 Citations (Scopus)

Abstract

Autophagy is a major route by which cytoplasmic contents are delivered to the lysosome for degradation. Many autophagyrelated (ATG) genes have been identified in yeast. Although most of them are conserved in human, the molecular composition of the Atg1 complex appears to differ between yeast and mammals. In yeast, Atg1 forms a complex with Atg11, Atg13, Atg17, Atg29 and Atg31, whereas mammalian Atg1 (ULK1/2) interacts with Atg13 and FIP200. Here, we identify a novel mammalian Atg13 binding protein, named Atg101. Atg101 shows no homology with other Atg proteins, and is conserved in various eukaryotes, but not in Saccharomyces cerevisiae. Atg101 associates with the ULK-Atg13-FIP200 complex, most likely through direct interaction with Atg13. In Atg13 siRNA-treated cells, Atg101 is present solely as a monomer. Interaction between Atg101 and the ULK-Atg13-FIP200 complex is stable, and is not regulated by nutrient conditions. GFP-Atg101 localizes to the isolation membrane/phagophore. GFP-LC3 dot formation is suppressed and endogenous LC3-I accumulates in Atg101 siRNA-treated cells, suggesting that Atg101 is a critical factor for autophagy. Furthermore, Atg101 is important for the stability and basal phosphorylation of Atg13 and ULK1. These data suggest that Atg101 is a novel Atg protein that functions together with ULK, Atg13 and FIP200.

Original languageEnglish
Pages (from-to)973-979
Number of pages7
JournalAutophagy
Volume5
Issue number7
DOIs
Publication statusPublished - 2009 Oct 1
Externally publishedYes

Fingerprint

Autophagy
Yeasts
Small Interfering RNA
Proteins
Lysosomes
Eukaryota
Saccharomyces cerevisiae
Mammals
Carrier Proteins
Phosphorylation
Food
Membranes
Genes

Keywords

  • Atg1
  • Atg10
  • Atg13
  • Autophagy
  • FIP200
  • RB1CC1
  • ULK

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Hosokawa, N., Sasaki, T., Iemura, S. I., Natsume, T., Hara, T., & Mizushima, N. (2009). Atg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy, 5(7), 973-979. https://doi.org/10.4161/auto.5.7.9296

Atg101, a novel mammalian autophagy protein interacting with Atg13. / Hosokawa, Nao; Sasaki, Takahiro; Iemura, Shun Ichiro; Natsume, Tohru; Hara, Taichi; Mizushima, Noboru.

In: Autophagy, Vol. 5, No. 7, 01.10.2009, p. 973-979.

Research output: Contribution to journalArticle

Hosokawa, N, Sasaki, T, Iemura, SI, Natsume, T, Hara, T & Mizushima, N 2009, 'Atg101, a novel mammalian autophagy protein interacting with Atg13', Autophagy, vol. 5, no. 7, pp. 973-979. https://doi.org/10.4161/auto.5.7.9296
Hosokawa N, Sasaki T, Iemura SI, Natsume T, Hara T, Mizushima N. Atg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy. 2009 Oct 1;5(7):973-979. https://doi.org/10.4161/auto.5.7.9296
Hosokawa, Nao ; Sasaki, Takahiro ; Iemura, Shun Ichiro ; Natsume, Tohru ; Hara, Taichi ; Mizushima, Noboru. / Atg101, a novel mammalian autophagy protein interacting with Atg13. In: Autophagy. 2009 ; Vol. 5, No. 7. pp. 973-979.
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