Atomic force microscope imaging of Escherichia coli cell using anti-E. coli antibody-conjugated probe (in aqueous) solutions

T. Tanaka, N. Nakamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Adhesion forces between bovine serum albumin (BSA) immobilized on gold substrate and an anti-BSA antibody conjugated on the gold coated probe of atomic force microscope (AFM) were determined by a force curve measurement. Specific adhesion forces were detected between the antibody-conjugated probe and immobilized BSA. The frequency of specific interactions was suppressed by adding free BSA. This result indicated that the curves originated from specific antigen-antibody interactions. On the basis of the above mentioned result, adhesion forces of an anti-Escherichia coli antibody known to recognize a protein located on the cell surface of E. coli were measured. E. coli cells were specifically detected by the measurement of adhesion forces between E. coli cells and the anti-E. coli antibody-conjugated probe. The AFM image of a single E. coli cell was also obtained using the anti-E. coli antibody-conjugated probe.

Original languageEnglish
Pages (from-to)3827-3832
Number of pages6
JournalElectrochimica Acta
Volume44
Issue number21
DOIs
Publication statusPublished - 1999 Jun 1
Externally publishedYes

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry

Fingerprint Dive into the research topics of 'Atomic force microscope imaging of Escherichia coli cell using anti-E. coli antibody-conjugated probe (in aqueous) solutions'. Together they form a unique fingerprint.

  • Cite this