Atomic force microscope imaging of Escherichia coli cell using anti-E. coli antibody-conjugated probe (in aqueous) solutions

T. Tanaka, N. Nakamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Adhesion forces between bovine serum albumin (BSA) immobilized on gold substrate and an anti-BSA antibody conjugated on the gold coated probe of atomic force microscope (AFM) were determined by a force curve measurement. Specific adhesion forces were detected between the antibody-conjugated probe and immobilized BSA. The frequency of specific interactions was suppressed by adding free BSA. This result indicated that the curves originated from specific antigen-antibody interactions. On the basis of the above mentioned result, adhesion forces of an anti-Escherichia coli antibody known to recognize a protein located on the cell surface of E. coli were measured. E. coli cells were specifically detected by the measurement of adhesion forces between E. coli cells and the anti-E. coli antibody-conjugated probe. The AFM image of a single E. coli cell was also obtained using the anti-E. coli antibody-conjugated probe.

Original languageEnglish
Pages (from-to)3827-3832
Number of pages6
JournalElectrochimica Acta
Volume44
Issue number21
DOIs
Publication statusPublished - 1999 Jun 1
Externally publishedYes

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Antibodies
Escherichia coli
Microscopes
Imaging techniques
Bovine Serum Albumin
Adhesion
Gold
Antigens
Proteins
Substrates

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry

Cite this

Atomic force microscope imaging of Escherichia coli cell using anti-E. coli antibody-conjugated probe (in aqueous) solutions. / Tanaka, T.; Nakamura, N.; Matsunaga, Tadashi.

In: Electrochimica Acta, Vol. 44, No. 21, 01.06.1999, p. 3827-3832.

Research output: Contribution to journalArticle

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