Atomic force microscopic observation of three-dimensional morphological changes of neurons when stimulated by a neurotransmitter

Tomoko Hosono, Mari Yamanaka, Takuro Tojima, Yukako Yamane, Hisayo Sadamoto, Dai Hatakeyama, Hisashi Haga, Kazushige Kawabata, Kazuhiro Abe, Etsuro Ito

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

As the first step in the study of morphological changes in neurons associated with their functional changes, we applied atomic force microscopy (AFM) for the observation of fine three-dimensional morphological changes in rat cerebellar granule cells stimulated by an agonist of glutamate receptors, N-methyl-D-aspartate (NMDA). The AFM revealed that NMDA changed the cross-sections of cell bodies from a trapezoid-like form to a triangle-like form within a minute. The fine hill-like structures on the top surfaces of the cell bodies became wider during the same period. These results were suggested to be induced by the depolymerization of filamentous actin triggered by the entry of Ca2+ via cation channels complexed with the activated NMDA receptors.

Original languageEnglish
Pages (from-to)3940-3945
Number of pages6
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume38
Issue number6 B
Publication statusPublished - 1999 Jun
Externally publishedYes

Fingerprint

neurotransmitters
aspartates
neurons
Neurons
Atomic force microscopy
Cells
Depolymerization
atomic force microscopy
trapezoids
Rats
depolymerization
glutamates
Positive ions
entry
triangles
rats
cations
cross sections
cells

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Atomic force microscopic observation of three-dimensional morphological changes of neurons when stimulated by a neurotransmitter. / Hosono, Tomoko; Yamanaka, Mari; Tojima, Takuro; Yamane, Yukako; Sadamoto, Hisayo; Hatakeyama, Dai; Haga, Hisashi; Kawabata, Kazushige; Abe, Kazuhiro; Ito, Etsuro.

In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Vol. 38, No. 6 B, 06.1999, p. 3940-3945.

Research output: Contribution to journalArticle

Hosono, Tomoko ; Yamanaka, Mari ; Tojima, Takuro ; Yamane, Yukako ; Sadamoto, Hisayo ; Hatakeyama, Dai ; Haga, Hisashi ; Kawabata, Kazushige ; Abe, Kazuhiro ; Ito, Etsuro. / Atomic force microscopic observation of three-dimensional morphological changes of neurons when stimulated by a neurotransmitter. In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers. 1999 ; Vol. 38, No. 6 B. pp. 3940-3945.
@article{b2e7c979ae9f41149ddcbd6bd3975c2c,
title = "Atomic force microscopic observation of three-dimensional morphological changes of neurons when stimulated by a neurotransmitter",
abstract = "As the first step in the study of morphological changes in neurons associated with their functional changes, we applied atomic force microscopy (AFM) for the observation of fine three-dimensional morphological changes in rat cerebellar granule cells stimulated by an agonist of glutamate receptors, N-methyl-D-aspartate (NMDA). The AFM revealed that NMDA changed the cross-sections of cell bodies from a trapezoid-like form to a triangle-like form within a minute. The fine hill-like structures on the top surfaces of the cell bodies became wider during the same period. These results were suggested to be induced by the depolymerization of filamentous actin triggered by the entry of Ca2+ via cation channels complexed with the activated NMDA receptors.",
author = "Tomoko Hosono and Mari Yamanaka and Takuro Tojima and Yukako Yamane and Hisayo Sadamoto and Dai Hatakeyama and Hisashi Haga and Kazushige Kawabata and Kazuhiro Abe and Etsuro Ito",
year = "1999",
month = "6",
language = "English",
volume = "38",
pages = "3940--3945",
journal = "Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes",
issn = "0021-4922",
publisher = "Japan Society of Applied Physics",
number = "6 B",

}

TY - JOUR

T1 - Atomic force microscopic observation of three-dimensional morphological changes of neurons when stimulated by a neurotransmitter

AU - Hosono, Tomoko

AU - Yamanaka, Mari

AU - Tojima, Takuro

AU - Yamane, Yukako

AU - Sadamoto, Hisayo

AU - Hatakeyama, Dai

AU - Haga, Hisashi

AU - Kawabata, Kazushige

AU - Abe, Kazuhiro

AU - Ito, Etsuro

PY - 1999/6

Y1 - 1999/6

N2 - As the first step in the study of morphological changes in neurons associated with their functional changes, we applied atomic force microscopy (AFM) for the observation of fine three-dimensional morphological changes in rat cerebellar granule cells stimulated by an agonist of glutamate receptors, N-methyl-D-aspartate (NMDA). The AFM revealed that NMDA changed the cross-sections of cell bodies from a trapezoid-like form to a triangle-like form within a minute. The fine hill-like structures on the top surfaces of the cell bodies became wider during the same period. These results were suggested to be induced by the depolymerization of filamentous actin triggered by the entry of Ca2+ via cation channels complexed with the activated NMDA receptors.

AB - As the first step in the study of morphological changes in neurons associated with their functional changes, we applied atomic force microscopy (AFM) for the observation of fine three-dimensional morphological changes in rat cerebellar granule cells stimulated by an agonist of glutamate receptors, N-methyl-D-aspartate (NMDA). The AFM revealed that NMDA changed the cross-sections of cell bodies from a trapezoid-like form to a triangle-like form within a minute. The fine hill-like structures on the top surfaces of the cell bodies became wider during the same period. These results were suggested to be induced by the depolymerization of filamentous actin triggered by the entry of Ca2+ via cation channels complexed with the activated NMDA receptors.

UR - http://www.scopus.com/inward/record.url?scp=0032594493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032594493&partnerID=8YFLogxK

M3 - Article

VL - 38

SP - 3940

EP - 3945

JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

SN - 0021-4922

IS - 6 B

ER -