Attachment of DNA to microfabricated arrays with self-assembled monolayer

Guo Jun Zhang, Takashi Tanii, Takeo Miyake, Takashi Funatsu, Iwao Ohdomari

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A novel approach of fabricating sub-10-μm patterns on silicon surfaces by electron beam (EB) lithography for attachment of oligonucleotides was described. The shape of the microfabricated arrays was observed to be regular by optical microscopy. An octadecyltrimethoxysilane (ODS) monolayer was deposited on the regions outside the patterned areas to minimize the nonspecific binding of biomolecules. Cy 5-labeled target DNA was hybridized to both complementary and noncomplementary oligonucleotides that were covalently anchored to micropatterns. As a result, the micropatterns where specific binding occurred show strong signals, whereas no signals are observed in the case of nonspecific binding. These data indicate that miniature micro- and nano-arrays will find applications in biochips and biosensors.

Original languageEnglish
Pages (from-to)452-455
Number of pages4
JournalThin Solid Films
Volume464-465
DOIs
Publication statusPublished - 2004 Oct

    Fingerprint

Keywords

  • Attachment
  • DNA
  • Electron beam lithography
  • Fabrication
  • Fluorescence detection
  • Micropatterns

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Condensed Matter Physics
  • Surfaces and Interfaces

Cite this