Attenuated food anticipatory activity and abnormal circadian locomotor rhythms in RGS16 knockdown mice

Naoto Hayasaka, Kazuyuki Aoki, Saori Kinoshita, Shoutaroh Yamaguchi, John K. Wakefield, Sachiyo Tsuji-Kawahara, Kazumasa Horikawa, Hiroshi Ikegami, Shigeharu Wakana, Takamichi Murakami, Ram Ramabhadran, Masaaki Miyazawa, Shigenobu Shibata

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Regulators of G protein signaling (RGS) are a multi-functional protein family, which functions in part as GTPase-activating proteins (GAPs) of G protein α-subunits to terminate G protein signaling. Previous studies have demonstrated that the Rgs16 transcripts exhibit robust circadian rhythms both in the suprachiasmatic nucleus (SCN), the master circadian light-entrainable oscillator (LEO) of the hypothalamus, and in the liver. To investigate the role of RGS16 in the circadian clock in vivo, we generated two independent transgenic mouse lines using lentiviral vectors expressing short hairpin RNA (shRNA) targeting the Rgs16 mRNA. The knockdown mice demonstrated significantly shorter free-running period of locomotor activity rhythms and reduced total activity as compared to the wild-type siblings. In addition, when feeding was restricted during the daytime, food-entrainable oscillator (FEO)-driven elevated food-anticipatory activity (FAA) observed prior to the scheduled feeding time was significantly attenuated in the knockdown mice. Whereas the restricted feeding phase-advanced the rhythmic expression of the Per2 clock gene in liver and thalamus in the wild-type animals, the above phase shift was not observed in the knockdown mice. This is the first in vivo demonstration that a common regulator of G protein signaling is involved in the two separate, but interactive circadian timing systems, LEO and FEO. The present study also suggests that liver and/or thalamus regulate the food-entrained circadian behavior through G protein-mediated signal transduction pathway(s).

    Original languageEnglish
    Article numbere17655
    JournalPLoS One
    Volume6
    Issue number3
    DOIs
    Publication statusPublished - 2011

    Fingerprint

    Circadian Rhythm
    G-proteins
    GTP-Binding Proteins
    GTP-Binding Protein Regulators
    Liver
    Food
    Circadian Clocks
    mice
    Thalamus
    thalamus
    Clocks
    circadian rhythm
    liver
    GTPase-Activating Proteins
    Light
    Signal transduction
    Suprachiasmatic Nucleus
    Wild Animals
    GTPase-activating proteins
    Protein Subunits

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Hayasaka, N., Aoki, K., Kinoshita, S., Yamaguchi, S., Wakefield, J. K., Tsuji-Kawahara, S., ... Shibata, S. (2011). Attenuated food anticipatory activity and abnormal circadian locomotor rhythms in RGS16 knockdown mice. PLoS One, 6(3), [e17655]. https://doi.org/10.1371/journal.pone.0017655

    Attenuated food anticipatory activity and abnormal circadian locomotor rhythms in RGS16 knockdown mice. / Hayasaka, Naoto; Aoki, Kazuyuki; Kinoshita, Saori; Yamaguchi, Shoutaroh; Wakefield, John K.; Tsuji-Kawahara, Sachiyo; Horikawa, Kazumasa; Ikegami, Hiroshi; Wakana, Shigeharu; Murakami, Takamichi; Ramabhadran, Ram; Miyazawa, Masaaki; Shibata, Shigenobu.

    In: PLoS One, Vol. 6, No. 3, e17655, 2011.

    Research output: Contribution to journalArticle

    Hayasaka, N, Aoki, K, Kinoshita, S, Yamaguchi, S, Wakefield, JK, Tsuji-Kawahara, S, Horikawa, K, Ikegami, H, Wakana, S, Murakami, T, Ramabhadran, R, Miyazawa, M & Shibata, S 2011, 'Attenuated food anticipatory activity and abnormal circadian locomotor rhythms in RGS16 knockdown mice', PLoS One, vol. 6, no. 3, e17655. https://doi.org/10.1371/journal.pone.0017655
    Hayasaka N, Aoki K, Kinoshita S, Yamaguchi S, Wakefield JK, Tsuji-Kawahara S et al. Attenuated food anticipatory activity and abnormal circadian locomotor rhythms in RGS16 knockdown mice. PLoS One. 2011;6(3). e17655. https://doi.org/10.1371/journal.pone.0017655
    Hayasaka, Naoto ; Aoki, Kazuyuki ; Kinoshita, Saori ; Yamaguchi, Shoutaroh ; Wakefield, John K. ; Tsuji-Kawahara, Sachiyo ; Horikawa, Kazumasa ; Ikegami, Hiroshi ; Wakana, Shigeharu ; Murakami, Takamichi ; Ramabhadran, Ram ; Miyazawa, Masaaki ; Shibata, Shigenobu. / Attenuated food anticipatory activity and abnormal circadian locomotor rhythms in RGS16 knockdown mice. In: PLoS One. 2011 ; Vol. 6, No. 3.
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