Attenuating effect of clock mutation on triglyceride contents in the ICR mouse liver under a high-fat diet

Takashi Kudo, Toru Tamagawa, Mihoko Kawashima, Natsuko Mito, Shigenobu Shibata

    Research output: Contribution to journalArticle

    57 Citations (Scopus)

    Abstract

    Energy homeostasis is subjected to a circadian control that synchronizes energy intake and expenditure. The transcription factor CLOCK, a key component of the molecular circadian clock, controls many kinds of rhythms, such as those for locomotor activity, body temperature, and metabolic functions. The purpose of the present study is to understand the function of the Clock gene during lipid metabolism in the liver using Clock-mutant mice. Clock-mutant mice with an ICR background were fed a high-fat diet for 13 weeks, and liver triglyceride, serum triglyceride, and serum free fatty acid levels were examined. Triglyceride content in the liver was significantly less increased in Clock-mutant mice on a high-fat diet compared to wild-type mice on a high-fat diet. Acsl4 and Fabp1 mRNA levels in the liver showed daily rhythms in wild-type mice. In contrast, Clock -mutant mice had attenuated daily rhythms of Acsl4 and Fabp1 gene expression in the liver under both normal and high-fat diet conditions compared to wild-type mice. In Clock-mutant mice, suppression of Acsl4 and Fabp1 mRNA in the liver under high-fat diet conditions may have attenuated the accumulation of triglycerides in the liver compared to wild-type mice under the same conditions. In conclusion, the authors demonstrate that mice with a Clock mutation showed less triglyceride accumulation in the liver through the suppression of Acsl4 and Fabp1 gene expression when fed a high-fat diet compared to wild-type mice fed the same diet.

    Original languageEnglish
    Pages (from-to)312-323
    Number of pages12
    JournalJournal of Biological Rhythms
    Volume22
    Issue number4
    DOIs
    Publication statusPublished - 2007 Aug

    Fingerprint

    Inbred ICR Mouse
    High Fat Diet
    high fat diet
    Triglycerides
    triacylglycerols
    mutation
    liver
    Mutation
    Liver
    mice
    mutants
    Gene Expression
    Messenger RNA
    Circadian Clocks
    gene expression
    Locomotion
    Energy Intake
    Body Temperature
    Serum
    Lipid Metabolism

    Keywords

    • Circadian rhythm
    • Clock gene
    • Clock mutant
    • High fat
    • Liver
    • Triglyceride

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences (miscellaneous)
    • Physiology
    • Physiology (medical)

    Cite this

    Attenuating effect of clock mutation on triglyceride contents in the ICR mouse liver under a high-fat diet. / Kudo, Takashi; Tamagawa, Toru; Kawashima, Mihoko; Mito, Natsuko; Shibata, Shigenobu.

    In: Journal of Biological Rhythms, Vol. 22, No. 4, 08.2007, p. 312-323.

    Research output: Contribution to journalArticle

    Kudo, Takashi ; Tamagawa, Toru ; Kawashima, Mihoko ; Mito, Natsuko ; Shibata, Shigenobu. / Attenuating effect of clock mutation on triglyceride contents in the ICR mouse liver under a high-fat diet. In: Journal of Biological Rhythms. 2007 ; Vol. 22, No. 4. pp. 312-323.
    @article{688af9d2f3f543558d3bc92ec28ebd4f,
    title = "Attenuating effect of clock mutation on triglyceride contents in the ICR mouse liver under a high-fat diet",
    abstract = "Energy homeostasis is subjected to a circadian control that synchronizes energy intake and expenditure. The transcription factor CLOCK, a key component of the molecular circadian clock, controls many kinds of rhythms, such as those for locomotor activity, body temperature, and metabolic functions. The purpose of the present study is to understand the function of the Clock gene during lipid metabolism in the liver using Clock-mutant mice. Clock-mutant mice with an ICR background were fed a high-fat diet for 13 weeks, and liver triglyceride, serum triglyceride, and serum free fatty acid levels were examined. Triglyceride content in the liver was significantly less increased in Clock-mutant mice on a high-fat diet compared to wild-type mice on a high-fat diet. Acsl4 and Fabp1 mRNA levels in the liver showed daily rhythms in wild-type mice. In contrast, Clock -mutant mice had attenuated daily rhythms of Acsl4 and Fabp1 gene expression in the liver under both normal and high-fat diet conditions compared to wild-type mice. In Clock-mutant mice, suppression of Acsl4 and Fabp1 mRNA in the liver under high-fat diet conditions may have attenuated the accumulation of triglycerides in the liver compared to wild-type mice under the same conditions. In conclusion, the authors demonstrate that mice with a Clock mutation showed less triglyceride accumulation in the liver through the suppression of Acsl4 and Fabp1 gene expression when fed a high-fat diet compared to wild-type mice fed the same diet.",
    keywords = "Circadian rhythm, Clock gene, Clock mutant, High fat, Liver, Triglyceride",
    author = "Takashi Kudo and Toru Tamagawa and Mihoko Kawashima and Natsuko Mito and Shigenobu Shibata",
    year = "2007",
    month = "8",
    doi = "10.1177/0748730407302625",
    language = "English",
    volume = "22",
    pages = "312--323",
    journal = "Journal of Biological Rhythms",
    issn = "0748-7304",
    publisher = "SAGE Publications Inc.",
    number = "4",

    }

    TY - JOUR

    T1 - Attenuating effect of clock mutation on triglyceride contents in the ICR mouse liver under a high-fat diet

    AU - Kudo, Takashi

    AU - Tamagawa, Toru

    AU - Kawashima, Mihoko

    AU - Mito, Natsuko

    AU - Shibata, Shigenobu

    PY - 2007/8

    Y1 - 2007/8

    N2 - Energy homeostasis is subjected to a circadian control that synchronizes energy intake and expenditure. The transcription factor CLOCK, a key component of the molecular circadian clock, controls many kinds of rhythms, such as those for locomotor activity, body temperature, and metabolic functions. The purpose of the present study is to understand the function of the Clock gene during lipid metabolism in the liver using Clock-mutant mice. Clock-mutant mice with an ICR background were fed a high-fat diet for 13 weeks, and liver triglyceride, serum triglyceride, and serum free fatty acid levels were examined. Triglyceride content in the liver was significantly less increased in Clock-mutant mice on a high-fat diet compared to wild-type mice on a high-fat diet. Acsl4 and Fabp1 mRNA levels in the liver showed daily rhythms in wild-type mice. In contrast, Clock -mutant mice had attenuated daily rhythms of Acsl4 and Fabp1 gene expression in the liver under both normal and high-fat diet conditions compared to wild-type mice. In Clock-mutant mice, suppression of Acsl4 and Fabp1 mRNA in the liver under high-fat diet conditions may have attenuated the accumulation of triglycerides in the liver compared to wild-type mice under the same conditions. In conclusion, the authors demonstrate that mice with a Clock mutation showed less triglyceride accumulation in the liver through the suppression of Acsl4 and Fabp1 gene expression when fed a high-fat diet compared to wild-type mice fed the same diet.

    AB - Energy homeostasis is subjected to a circadian control that synchronizes energy intake and expenditure. The transcription factor CLOCK, a key component of the molecular circadian clock, controls many kinds of rhythms, such as those for locomotor activity, body temperature, and metabolic functions. The purpose of the present study is to understand the function of the Clock gene during lipid metabolism in the liver using Clock-mutant mice. Clock-mutant mice with an ICR background were fed a high-fat diet for 13 weeks, and liver triglyceride, serum triglyceride, and serum free fatty acid levels were examined. Triglyceride content in the liver was significantly less increased in Clock-mutant mice on a high-fat diet compared to wild-type mice on a high-fat diet. Acsl4 and Fabp1 mRNA levels in the liver showed daily rhythms in wild-type mice. In contrast, Clock -mutant mice had attenuated daily rhythms of Acsl4 and Fabp1 gene expression in the liver under both normal and high-fat diet conditions compared to wild-type mice. In Clock-mutant mice, suppression of Acsl4 and Fabp1 mRNA in the liver under high-fat diet conditions may have attenuated the accumulation of triglycerides in the liver compared to wild-type mice under the same conditions. In conclusion, the authors demonstrate that mice with a Clock mutation showed less triglyceride accumulation in the liver through the suppression of Acsl4 and Fabp1 gene expression when fed a high-fat diet compared to wild-type mice fed the same diet.

    KW - Circadian rhythm

    KW - Clock gene

    KW - Clock mutant

    KW - High fat

    KW - Liver

    KW - Triglyceride

    UR - http://www.scopus.com/inward/record.url?scp=34447579941&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=34447579941&partnerID=8YFLogxK

    U2 - 10.1177/0748730407302625

    DO - 10.1177/0748730407302625

    M3 - Article

    C2 - 17660448

    AN - SCOPUS:34447579941

    VL - 22

    SP - 312

    EP - 323

    JO - Journal of Biological Rhythms

    JF - Journal of Biological Rhythms

    SN - 0748-7304

    IS - 4

    ER -