Attomolar detection of influenza A virus hemagglutinin human H1 and avian H5 using glycan-blotted field effect transistor biosensor

Sho Hideshima, Hiroshi Hinou, Daisuke Ebihara, Ryosuke Sato, Shigeki Kuroiwa, Takuya Nakanishi, Shin Ichiro Nishimura, Tetsuya Osaka

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Influenza virus, through cell invasion and propagation with the interaction between hemagglutinin (HA) present on its surface and glycans on the host cell, causes a rapidly spreading infection throughout the world. In the present investigation, we succeeded for the first time in the attomolar-level sensing and discrimination of influenza A viral HA molecules H1 and H5 by using a glycan-immobilized field effect transistor (FET) biosensor. The small ligand glycans immobilized on the FET device, which make effective use of the charge-detectable region for FET-based detection in terms of Debye length, gave an advantage in the highly sensitive detection of the proteins. Two kinds of trisaccharides receptors terminating in sialic acid-α2,6-galactose (6′-sialyllactose) and in sialic acid-α2,3-galactose (3′-sialyllactose) were conjugated directly with the SiO2 surface of FET devices by a simple glycoblotting method using the self-assembled monolayer (SAM) of aminooxy terminated silane-coupling reagent, 3-aminooxypropyltriethoxysilane. Furthermore, it was demonstrated that the FETs with densely immobilized glycans, which possess the high capture ability by achieving the glycoside cluster effect, clearly distinguish HA molecules between their subtypes H1 (human) and H5 (avian) at the attomolar level, while the conventional method based on HA antibodies achieves only picomolar-level detection. Our findings indicate that the glycan-immobilized FET is a promising device to detect various pathogenic bacteria and viruses through glycan-protein interaction found ubiquitously in many infectious diseases.

Original languageEnglish
Pages (from-to)5641-5644
Number of pages4
JournalAnalytical Chemistry
Volume85
Issue number12
DOIs
Publication statusPublished - 2013 Jun 18

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Field effect transistors
Biosensors
Polysaccharides
Hemagglutinins
N-Acetylneuraminic Acid
Galactose
Viruses
Viral Hemagglutinins
Trisaccharides
Silanes
Molecules
Self assembled monolayers
Glycosides
human influenza A virus hemagglutinin
Bacteria
Proteins
Ligands
Antibodies

ASJC Scopus subject areas

  • Analytical Chemistry

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Attomolar detection of influenza A virus hemagglutinin human H1 and avian H5 using glycan-blotted field effect transistor biosensor. / Hideshima, Sho; Hinou, Hiroshi; Ebihara, Daisuke; Sato, Ryosuke; Kuroiwa, Shigeki; Nakanishi, Takuya; Nishimura, Shin Ichiro; Osaka, Tetsuya.

In: Analytical Chemistry, Vol. 85, No. 12, 18.06.2013, p. 5641-5644.

Research output: Contribution to journalArticle

Hideshima, Sho ; Hinou, Hiroshi ; Ebihara, Daisuke ; Sato, Ryosuke ; Kuroiwa, Shigeki ; Nakanishi, Takuya ; Nishimura, Shin Ichiro ; Osaka, Tetsuya. / Attomolar detection of influenza A virus hemagglutinin human H1 and avian H5 using glycan-blotted field effect transistor biosensor. In: Analytical Chemistry. 2013 ; Vol. 85, No. 12. pp. 5641-5644.
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