Aurora-B regulates the cleavage furrow-specific vimentin phosphorylation in the cytokinetic process

Hidemasa Goto, Yoshihiro Yasui, Aie Kawajiri, Erich A. Nigg, Yasuhiko Terada, Masaaki Tatsuka, Koh ichi Nagata, Masaki Inagaki

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Aurora-B is an evolutionally conserved protein kinase that regulates several mitotic events including cytokinesis. We previously demonstrated the possible existence of a protein kinase that phosphorylates at least Ser-72 on vimentin, the most widely expressed intermediate filament protein, in the cleavage furrow-specific manner. Here we showed that vimentin-Ser-72 phosphorylation occurred specifically at the border of the Aurora-B-localized area from anaphase to telophase. Expression of a dominant-negative mutant of Aurora-B led to a reduction of this vimentin-Ser-72 phosphorylation. In vitro analyses revealed that Aurora-B phosphorylates vimentin at ∼2 mol phosphate/mol of substrate for 30 min and that this phosphorylation dramatically inhibits vimentin filament formation. We further identified eight Aurora-B phosphorylation sites, including Ser-72 on vimentin, and then constructed the mutant vimentin in which these identified sites are changed into Ala. Cells expressing this mutant formed an unusually long bridge-like intermediate filament structure between unseparated daughter cells. We then identified important phosphorylation sites for the bridge phenotype. Our findings indicate that Aurora-B regulates the cleavage furrow-specific vimentin phosphorylation and controls vimentin filament segregation in cytokinetic process.

Original languageEnglish
Pages (from-to)8526-8530
Number of pages5
JournalJournal of Biological Chemistry
Volume278
Issue number10
DOIs
Publication statusPublished - 2003 Mar 7
Externally publishedYes

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Phosphorylation
Vimentin
Protein Kinases
Telophase
Intermediate Filament Proteins
Anaphase
Cytokinesis
Intermediate Filaments
Phosphates
Cells
Phenotype

ASJC Scopus subject areas

  • Biochemistry

Cite this

Aurora-B regulates the cleavage furrow-specific vimentin phosphorylation in the cytokinetic process. / Goto, Hidemasa; Yasui, Yoshihiro; Kawajiri, Aie; Nigg, Erich A.; Terada, Yasuhiko; Tatsuka, Masaaki; Nagata, Koh ichi; Inagaki, Masaki.

In: Journal of Biological Chemistry, Vol. 278, No. 10, 07.03.2003, p. 8526-8530.

Research output: Contribution to journalArticle

Goto, H, Yasui, Y, Kawajiri, A, Nigg, EA, Terada, Y, Tatsuka, M, Nagata, KI & Inagaki, M 2003, 'Aurora-B regulates the cleavage furrow-specific vimentin phosphorylation in the cytokinetic process', Journal of Biological Chemistry, vol. 278, no. 10, pp. 8526-8530. https://doi.org/10.1074/jbc.M210892200
Goto, Hidemasa ; Yasui, Yoshihiro ; Kawajiri, Aie ; Nigg, Erich A. ; Terada, Yasuhiko ; Tatsuka, Masaaki ; Nagata, Koh ichi ; Inagaki, Masaki. / Aurora-B regulates the cleavage furrow-specific vimentin phosphorylation in the cytokinetic process. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 10. pp. 8526-8530.
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