Aurora-B/AIM-1 regulates the dynamic behavior of HP1α at the G 2-M transition

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Abstract

Heterochromatin protein 1 (HP1) plays an important role in heterochromatin formation and undergoes large-scale, progressive dissociation from heterochromatin in prophase cells. However, the mechanisms regulating the dynamic behavior of HP1 are poorly understood. In this study, the role of Aurora-B was investigated with respect to the dynamic behavior of HP1α. Mammalian Aurora-B, AIM-1, colocalizes with HP1α to the heterochromatin in G2. Depletion of Aurora-B/AIM-1 inhibited dissociation of HP1α from the chromosome arms at the G2-M transition. In addition, depletion of INCENP led to aberrant cellular localization of Aurora-B/AIM-1, but it did not affect heterochromatin targeting of HP1α. It was proposed in the binary switch hypothesis that phosphorylation of histone H3 at Ser-10 negatively regulates the binding of HP1α to the adjacent methylated Lys-9. However, Aurora-B/AIM-1-mediated phosphorylation of H3 induced dissociation of the HP1α chromodomain but not of the intact protein in vitro, indicating that the center and/or C-terminal domain of HP1α interferes with the effect of H3 phosphorylation on HP1α dissociation. Interestingly, Lys-9 methyltransferase SUV39H1 is abnormally localized together along the metaphase chromosome arms in Aurora-B/AIM-1-depleted cells. In conclusion, these results showed that Aurora-B/AIM-1 is necessary for regulated histone modifications involved in binding of HP1α by the N terminus of histone H3 during mitosis.

Original languageEnglish
Pages (from-to)3232-3241
Number of pages10
JournalMolecular Biology of the Cell
Volume17
Issue number7
DOIs
Publication statusPublished - 2006 Jul
Externally publishedYes

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Heterochromatin
Phosphorylation
Histones
Carrier Proteins
heterochromatin-specific nonhistone chromosomal protein HP-1
Histone Code
Chromosomes
Prophase
Methyltransferases
Metaphase
Mitosis
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Aurora-B/AIM-1 regulates the dynamic behavior of HP1α at the G 2-M transition. / Terada, Yasuhiko.

In: Molecular Biology of the Cell, Vol. 17, No. 7, 07.2006, p. 3232-3241.

Research output: Contribution to journalArticle

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