Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles

Hiroyuki Ota, Tae Kyu Lim, Tsuyoshi Tanaka, Tomoko Yoshino, Manabu Harada, Tadashi Matsunaga

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A novel, automated system, PNE-1080, equipped with eight automated pestle units and a spectrophotometer was developed for genomic DNA extraction from maize using aminosilane-modified bacterial magnetic particles (BMPs). The use of aminosilane-modified BMPs allowed highly accurate DNA recovery. The (A260-A320):(A280-A320) ratio of the extracted DNA was 1.9 ± 0.1. The DNA quality was sufficiently pure for PCR analysis. The PNE-1080 offered rapid assay completion (30 min) with high accuracy. Furthermore, the results of real-time PCR confirmed that our proposed method permitted the accurate determination of genetically modified DNA composition and correlated well with results obtained by conventional cetyltrimethylammonium bromide (CTAB)-based methods.

Original languageEnglish
Pages (from-to)361-368
Number of pages8
JournalJournal of Biotechnology
Volume125
Issue number3
DOIs
Publication statusPublished - 2006 Sep 18
Externally publishedYes

Fingerprint

Zea mays
DNA
Spectrophotometers
Real-Time Polymerase Chain Reaction
Assays
Recovery
Polymerase Chain Reaction
Chemical analysis

Keywords

  • Aminosilane-modified bacterial magnetic particles
  • DNA extraction
  • Genetically modified maize

ASJC Scopus subject areas

  • Biotechnology

Cite this

Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles. / Ota, Hiroyuki; Lim, Tae Kyu; Tanaka, Tsuyoshi; Yoshino, Tomoko; Harada, Manabu; Matsunaga, Tadashi.

In: Journal of Biotechnology, Vol. 125, No. 3, 18.09.2006, p. 361-368.

Research output: Contribution to journalArticle

Ota, Hiroyuki ; Lim, Tae Kyu ; Tanaka, Tsuyoshi ; Yoshino, Tomoko ; Harada, Manabu ; Matsunaga, Tadashi. / Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles. In: Journal of Biotechnology. 2006 ; Vol. 125, No. 3. pp. 361-368.
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