Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles

Hiroyuki Ota, Tae Kyu Lim, Tsuyoshi Tanaka, Tomoko Yoshino, Manabu Harada, Tadashi Matsunaga

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A novel, automated system, PNE-1080, equipped with eight automated pestle units and a spectrophotometer was developed for genomic DNA extraction from maize using aminosilane-modified bacterial magnetic particles (BMPs). The use of aminosilane-modified BMPs allowed highly accurate DNA recovery. The (A260-A320):(A280-A320) ratio of the extracted DNA was 1.9 ± 0.1. The DNA quality was sufficiently pure for PCR analysis. The PNE-1080 offered rapid assay completion (30 min) with high accuracy. Furthermore, the results of real-time PCR confirmed that our proposed method permitted the accurate determination of genetically modified DNA composition and correlated well with results obtained by conventional cetyltrimethylammonium bromide (CTAB)-based methods.

Original languageEnglish
Pages (from-to)361-368
Number of pages8
JournalJournal of Biotechnology
Volume125
Issue number3
DOIs
Publication statusPublished - 2006 Sep 18
Externally publishedYes

Keywords

  • Aminosilane-modified bacterial magnetic particles
  • DNA extraction
  • Genetically modified maize

ASJC Scopus subject areas

  • Biotechnology

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