Axle-less F1-ATPase rotates in the correct direction

Shou Furuike, Mohammad Delawar Hossain, Yasushi Maki, Kengo Adachi, Toshiharu Suzuki, Ayako Kohori, Hiroyasu Itoh, Masasuke Yoshida, Kazuhiko Kinosita

    Research output: Contribution to journalArticle

    86 Citations (Scopus)

    Abstract

    F1-adenosine triphosphatase (ATPase) is an ATP-driven rotary molecular motor in which the central γ subunit rotates inside a cylinder made of three α and three β subunits alternately arranged. The rotor shaft, an antiparallel α-helical coiled coil of the amino and carboxyl termini of the γ subunit, deeply penetrates the central cavity of the stator cylinder. We truncated the shaft step by step until the remaining rotor head would be outside the cavity and simply sat on the concave entrance of the stator orifice. All truncation mutants rotated in the correct direction, implying torque generation, although the average rotary speeds were low and short mutants exhibited moments of irregular motion. Neither a fixed pivot nor a rigid axle was needed for rotation of F1-ATPase.

    Original languageEnglish
    Pages (from-to)955-958
    Number of pages4
    JournalScience
    Volume319
    Issue number5865
    DOIs
    Publication statusPublished - 2008 Feb 15

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    Adenosine Triphosphatases
    Torque
    Adenosine Triphosphate
    Head
    Direction compound

    ASJC Scopus subject areas

    • General
    • Medicine(all)

    Cite this

    Furuike, S., Hossain, M. D., Maki, Y., Adachi, K., Suzuki, T., Kohori, A., ... Kinosita, K. (2008). Axle-less F1-ATPase rotates in the correct direction. Science, 319(5865), 955-958. https://doi.org/10.1126/science.1151343

    Axle-less F1-ATPase rotates in the correct direction. / Furuike, Shou; Hossain, Mohammad Delawar; Maki, Yasushi; Adachi, Kengo; Suzuki, Toshiharu; Kohori, Ayako; Itoh, Hiroyasu; Yoshida, Masasuke; Kinosita, Kazuhiko.

    In: Science, Vol. 319, No. 5865, 15.02.2008, p. 955-958.

    Research output: Contribution to journalArticle

    Furuike, S, Hossain, MD, Maki, Y, Adachi, K, Suzuki, T, Kohori, A, Itoh, H, Yoshida, M & Kinosita, K 2008, 'Axle-less F1-ATPase rotates in the correct direction', Science, vol. 319, no. 5865, pp. 955-958. https://doi.org/10.1126/science.1151343
    Furuike S, Hossain MD, Maki Y, Adachi K, Suzuki T, Kohori A et al. Axle-less F1-ATPase rotates in the correct direction. Science. 2008 Feb 15;319(5865):955-958. https://doi.org/10.1126/science.1151343
    Furuike, Shou ; Hossain, Mohammad Delawar ; Maki, Yasushi ; Adachi, Kengo ; Suzuki, Toshiharu ; Kohori, Ayako ; Itoh, Hiroyasu ; Yoshida, Masasuke ; Kinosita, Kazuhiko. / Axle-less F1-ATPase rotates in the correct direction. In: Science. 2008 ; Vol. 319, No. 5865. pp. 955-958.
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    AU - Suzuki, Toshiharu

    AU - Kohori, Ayako

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