Bacterial adhesion to and viability on positively charged polymer surfaces

Akihiko Terada, Atsushi Yuasa, Takashi Kushimoto, Satoshi Tsuneda, Akio Katakai, Masao Tamada

    Research output: Contribution to journalArticle

    97 Citations (Scopus)

    Abstract

    Secondary and tertiary amino groups were introduced into polymer chains grafted onto a polyethylene flat-sheet membrane to evaluate the effects of surface properties on the adhesion and viability of a strain of the Gram-negative bacterium Escherichia coli and a strain of the Gram-positive bacterium Bacillus subtilis. The characterization of the surfaces containing amino groups, i.e. ethylamino (EA) and diethylamino (DEA) groups, revealed that the membrane potentials are proportional to amino-group densities and contact angle hysteresis. A high bacterial adhesion rate constant k was observed at high membrane potential, which indicates that membrane potential could be used as an indicator for estimating bacterial adhesion to the EA and DEA sheets, especially in B. subtilis. The bacterial adhesion rate constant of E. coli markedly increased at a membrane potential higher than -7.8 mV, whereas that of B. subtilis increased at a membrane potential higher than -8.3 mV, at which the dominant effect on bacterial adhesion is expected to change. The viability experiments revealed that approximately 80% of E. coli cells adhering to the sheets with high membrane potential were inactivated after a contact time of 8 h, whereas 60% of B. subtilis cells were inactivated. Furthermore, E. coli viability significantly decreased at a membrane potential higher than -8 mV, whereas B. subtilis viability decreased as membrane potential increased, which reflects differences in cell wall structure between E. coli and B. subtilis.

    Original languageEnglish
    Pages (from-to)3575-3583
    Number of pages9
    JournalMicrobiology
    Volume152
    Issue number12
    DOIs
    Publication statusPublished - 2006 Dec

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    Bacterial Adhesion
    Microbial Viability
    Membrane Potentials
    Polymers
    Bacillus subtilis
    Escherichia coli
    Surface Properties
    Gram-Positive Bacteria
    Polyethylene
    Gram-Negative Bacteria
    Cell Wall
    Membranes

    ASJC Scopus subject areas

    • Microbiology

    Cite this

    Terada, A., Yuasa, A., Kushimoto, T., Tsuneda, S., Katakai, A., & Tamada, M. (2006). Bacterial adhesion to and viability on positively charged polymer surfaces. Microbiology, 152(12), 3575-3583. https://doi.org/10.1099/mic.0.28881-0

    Bacterial adhesion to and viability on positively charged polymer surfaces. / Terada, Akihiko; Yuasa, Atsushi; Kushimoto, Takashi; Tsuneda, Satoshi; Katakai, Akio; Tamada, Masao.

    In: Microbiology, Vol. 152, No. 12, 12.2006, p. 3575-3583.

    Research output: Contribution to journalArticle

    Terada, A, Yuasa, A, Kushimoto, T, Tsuneda, S, Katakai, A & Tamada, M 2006, 'Bacterial adhesion to and viability on positively charged polymer surfaces', Microbiology, vol. 152, no. 12, pp. 3575-3583. https://doi.org/10.1099/mic.0.28881-0
    Terada, Akihiko ; Yuasa, Atsushi ; Kushimoto, Takashi ; Tsuneda, Satoshi ; Katakai, Akio ; Tamada, Masao. / Bacterial adhesion to and viability on positively charged polymer surfaces. In: Microbiology. 2006 ; Vol. 152, No. 12. pp. 3575-3583.
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