Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System

Satoru Kurokawa, Jun Kabayama, Tsuguaki Fukuyasu, Seong Don Hwang, Chan Il Park, Seong Bin Park, Carmelo S. del Castillo, Jun ichi Hikima, Tae Sung Jung, Hidehiro Kondo, Ikuo Hirono, Haruko Takeyama, Takashi Aoki

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Mycobacterium marinum is difficult to distinguish from other species of Mycobacterium isolated from fish using biochemical methods. Here, we used genetic and proteomic analyses to distinguish three Mycobacterium strains: M. marinum strains MB2 and Europe were isolated from tropical and marine fish in Thailand and Europe, and Mycobacterium sp. 012931 strain was isolated from yellowtail in Japan. In phylogenetic trees based on gyrB, rpoB, and Ag85B genes, Mycobacterium sp. 012931 clustered with M. marinum strains MB2 and Europe, but in trees based on 16S rRNA, hsp65, and Ag85A genes Mycobacterium sp. 012931 did not cluster with the other strains. In proteomic analyses using a Bruker matrix-assisted laser desorption ionization Biotyper, the mass profile of Mycobacterium sp. 012931 differed from the mass profiles of the other two fish M. marinum strains. Therefore, Mycobacterium sp. 012931 is similar to M. marinum but is not the same, suggesting that it could be a subspecies of M. marinum.

    Original languageEnglish
    Pages (from-to)340-348
    Number of pages9
    JournalMarine Biotechnology
    Volume15
    Issue number3
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    Mycobacterium marinum
    Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
    Mycobacterium
    Fishes
    proteomics
    taxonomy
    phylogenetics
    phylogeny
    fish
    gene
    subspecies
    desorption
    Proteomics
    ionization
    laser
    matrix
    tropical fish
    Thailand
    Europe
    marine fish

    Keywords

    • MALDI Biotyper
    • Mycobacterium marinum
    • Mycobacterium sp.
    • Phylogenetic analysis

    ASJC Scopus subject areas

    • Aquatic Science
    • Biotechnology

    Cite this

    Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System. / Kurokawa, Satoru; Kabayama, Jun; Fukuyasu, Tsuguaki; Hwang, Seong Don; Park, Chan Il; Park, Seong Bin; del Castillo, Carmelo S.; Hikima, Jun ichi; Jung, Tae Sung; Kondo, Hidehiro; Hirono, Ikuo; Takeyama, Haruko; Aoki, Takashi.

    In: Marine Biotechnology, Vol. 15, No. 3, 2013, p. 340-348.

    Research output: Contribution to journalArticle

    Kurokawa, S, Kabayama, J, Fukuyasu, T, Hwang, SD, Park, CI, Park, SB, del Castillo, CS, Hikima, JI, Jung, TS, Kondo, H, Hirono, I, Takeyama, H & Aoki, T 2013, 'Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System', Marine Biotechnology, vol. 15, no. 3, pp. 340-348. https://doi.org/10.1007/s10126-012-9492-x
    Kurokawa, Satoru ; Kabayama, Jun ; Fukuyasu, Tsuguaki ; Hwang, Seong Don ; Park, Chan Il ; Park, Seong Bin ; del Castillo, Carmelo S. ; Hikima, Jun ichi ; Jung, Tae Sung ; Kondo, Hidehiro ; Hirono, Ikuo ; Takeyama, Haruko ; Aoki, Takashi. / Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System. In: Marine Biotechnology. 2013 ; Vol. 15, No. 3. pp. 340-348.
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