Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System

Satoru Kurokawa, Jun Kabayama, Tsuguaki Fukuyasu, Seong Don Hwang, Chan Il Park, Seong Bin Park, Carmelo S. del Castillo, Jun ichi Hikima, Tae Sung Jung, Hidehiro Kondo, Ikuo Hirono, Haruko Takeyama, Takashi Aoki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Mycobacterium marinum is difficult to distinguish from other species of Mycobacterium isolated from fish using biochemical methods. Here, we used genetic and proteomic analyses to distinguish three Mycobacterium strains: M. marinum strains MB2 and Europe were isolated from tropical and marine fish in Thailand and Europe, and Mycobacterium sp. 012931 strain was isolated from yellowtail in Japan. In phylogenetic trees based on gyrB, rpoB, and Ag85B genes, Mycobacterium sp. 012931 clustered with M. marinum strains MB2 and Europe, but in trees based on 16S rRNA, hsp65, and Ag85A genes Mycobacterium sp. 012931 did not cluster with the other strains. In proteomic analyses using a Bruker matrix-assisted laser desorption ionization Biotyper, the mass profile of Mycobacterium sp. 012931 differed from the mass profiles of the other two fish M. marinum strains. Therefore, Mycobacterium sp. 012931 is similar to M. marinum but is not the same, suggesting that it could be a subspecies of M. marinum.

Original languageEnglish
Pages (from-to)340-348
Number of pages9
JournalMarine Biotechnology
Volume15
Issue number3
DOIs
Publication statusPublished - 2013 Jan 1

Keywords

  • MALDI Biotyper
  • Mycobacterium marinum
  • Mycobacterium sp.
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

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    Kurokawa, S., Kabayama, J., Fukuyasu, T., Hwang, S. D., Park, C. I., Park, S. B., del Castillo, C. S., Hikima, J. I., Jung, T. S., Kondo, H., Hirono, I., Takeyama, H., & Aoki, T. (2013). Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System. Marine Biotechnology, 15(3), 340-348. https://doi.org/10.1007/s10126-012-9492-x