Bacterial collagen-binding domain targets undertwisted regions of collagen

Sagaya Theresa Leena Philominathan, Takaki Koide, Osamu Matsushita, Joshua Sakon

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    Clostridium histolyticum collagenase causes extensive degradation of collagen in connective tissue that results in gas gangrene. The C-terminal collagen-binding domain (CBD) of these enzymes is the minimal segment required to bind to a collagen fibril. CBD binds unidirectionally to the undertwisted C-terminus of triple helical collagen. Here, we examine whether CBD could also target undertwisted regions even in the middle of the triple helix. Collageneous peptides with an additional undertwisted region were synthesized by introducing a Gly → Ala substitution [(POG)xPOA(POG)y] 3, where x + y = 9 and x > 3). 1H-15N heteronuclear single quantum coherence nuclear magnetic resonance (HSQC NMR) titration studies with 15N-labeled CBD demonstrated that the minicollagen binds to a 10 Å wide 25 Å long cleft. Six collagenous peptides each labeled with a nitroxide radical were then titrated with 15N-labeled CBD. CBD binds to either the Gly → Ala substitution site or to the C-terminus of each minicollagen. Small-angle X-ray scattering measurements revealed that CBD prefers to bind the Gly → Ala site to the C-terminus. The HSQC NMR spectra of 15N-labeled minicollagen and minicollagen with undertwisted regions were unaffected by the titration of unlabeled CBD. The results imply that CBD binds to the undertwisted region of the minicollagen but does not actively unwind the triple helix.

    Original languageEnglish
    Pages (from-to)1554-1565
    Number of pages12
    JournalProtein Science
    Volume21
    Issue number10
    DOIs
    Publication statusPublished - 2012 Oct

    Fingerprint

    Collagen
    Titration
    Microbial Collagenase
    Substitution reactions
    Magnetic Resonance Spectroscopy
    Nuclear magnetic resonance
    Gas Gangrene
    Peptides
    X ray scattering
    Connective Tissue
    Gases
    X-Rays
    Tissue
    Degradation
    Enzymes

    Keywords

    • Binding
    • Collagen
    • NMR
    • Triple-helix
    • Undertwisted collagen

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

    Cite this

    Bacterial collagen-binding domain targets undertwisted regions of collagen. / Philominathan, Sagaya Theresa Leena; Koide, Takaki; Matsushita, Osamu; Sakon, Joshua.

    In: Protein Science, Vol. 21, No. 10, 10.2012, p. 1554-1565.

    Research output: Contribution to journalArticle

    Philominathan, STL, Koide, T, Matsushita, O & Sakon, J 2012, 'Bacterial collagen-binding domain targets undertwisted regions of collagen', Protein Science, vol. 21, no. 10, pp. 1554-1565. https://doi.org/10.1002/pro.2145
    Philominathan, Sagaya Theresa Leena ; Koide, Takaki ; Matsushita, Osamu ; Sakon, Joshua. / Bacterial collagen-binding domain targets undertwisted regions of collagen. In: Protein Science. 2012 ; Vol. 21, No. 10. pp. 1554-1565.
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