Bacterial inactivation by applying an alternating electromagnetic field using PAMAM dendron-modified magnetic nanoparticles

Atsushi Arakaki, Mami Takahashi, Masahito Hosokawa, Tadashi Matsunaga, Tsuyoshi Tanaka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this study, a method involving polyamidoamine dendron-modified magnetic nanoparticles (PAMAM-MNPs) along with application of an alternating magnetic field (AMF) was developed for inactivation of bacteria in water samples. The PAMAM-MNPs efficiently bound to Escherichia coli cells, resulting in magnetic recovery of cells from aqueous solutions. By applying the AMF (5kW, 250 kHz) to the cell suspension, E. coli cells were successfully inactivated within 10 min in the presence of the MNPs, while no effect was observed in the absence of the MNPs. The use of PAMAM-MNPs could increase the inactivation rate of E. coli under the applied AMF. E. coli cells with PAMAM-MNPs stained by propidium iodide (PI) exhibited apparent fluorescence after exposure to the AMF, suggesting the occurrence of membrane damage in the cells because of direct heat transfer from the PAMAM-MNPs. Our technique can be used to address bacterial contamination with wide varieties of microorganisms in water samples.

Original languageEnglish
Pages (from-to)324-327
Number of pages4
JournalElectrochemistry
Volume84
Issue number5
DOIs
Publication statusPublished - 2016 May 1
Externally publishedYes

Fingerprint

Electromagnetic fields
Escherichia coli
Nanoparticles
Magnetic fields
Water
Propidium
Microorganisms
Suspensions
Bacteria
Contamination
Fluorescence
PAMAM Starburst
dendron
Heat transfer
Membranes
Recovery

Keywords

  • Alternating magnetic field (AMF)
  • Bacterial inactivation
  • Magnetic nanoparticles (MNPs)
  • PAMAM-MNPs

ASJC Scopus subject areas

  • Electrochemistry

Cite this

Bacterial inactivation by applying an alternating electromagnetic field using PAMAM dendron-modified magnetic nanoparticles. / Arakaki, Atsushi; Takahashi, Mami; Hosokawa, Masahito; Matsunaga, Tadashi; Tanaka, Tsuyoshi.

In: Electrochemistry, Vol. 84, No. 5, 01.05.2016, p. 324-327.

Research output: Contribution to journalArticle

Arakaki, Atsushi ; Takahashi, Mami ; Hosokawa, Masahito ; Matsunaga, Tadashi ; Tanaka, Tsuyoshi. / Bacterial inactivation by applying an alternating electromagnetic field using PAMAM dendron-modified magnetic nanoparticles. In: Electrochemistry. 2016 ; Vol. 84, No. 5. pp. 324-327.
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