Bacterial ribonuclease P reaction is affected by substrate shape and magnesium ion concentration.

Tomoaki Ando, Terumichi Tanaka, Yo Kikuchi

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Bacterial RNase P is a ribonucleoprotein enzyme which cleaves 5'-precursor sequence of pre-tRNA for pre-tRNA maturation. The RNA component of bacterial RNase P is ribozyme. It recognizes cloverleaf shaped pre-tRNA and hairpin RNA with a CCA-3' tag sequence as its substrates. Previously, we reported that the substrate recognition of the E. coli RNase P ribozyme depends on the concentration of magnesium ion in vitro. In this report, we examined the substrate shape preference of the Bacillus subtilis RNase P ribozyme and compared it with that of the E. coli ribozyme. The results of the B. subtilis ribozyme displayed same tendency as the E. coli ribozyme. We also examined the effect of the protein component of the E. coli RNase P. Under the conditions tested, magnesium ion concentration dependency to substrate shape recognition was not observed when the holo enzyme was used.

Original languageEnglish
Title of host publicationNucleic acids research. Supplement (2001)
Pages293-294
Number of pages2
Edition3
Publication statusPublished - 2003
Externally publishedYes

Fingerprint

Ribonuclease P
Catalytic RNA
Magnesium
Ions
RNA Precursors
Escherichia coli
Bacillus subtilis
Bacterial RNA
Ribonucleoproteins
Escherichia coli Proteins
Enzymes
RNA

Cite this

Ando, T., Tanaka, T., & Kikuchi, Y. (2003). Bacterial ribonuclease P reaction is affected by substrate shape and magnesium ion concentration. In Nucleic acids research. Supplement (2001) (3 ed., pp. 293-294)

Bacterial ribonuclease P reaction is affected by substrate shape and magnesium ion concentration. / Ando, Tomoaki; Tanaka, Terumichi; Kikuchi, Yo.

Nucleic acids research. Supplement (2001). 3. ed. 2003. p. 293-294.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ando, T, Tanaka, T & Kikuchi, Y 2003, Bacterial ribonuclease P reaction is affected by substrate shape and magnesium ion concentration. in Nucleic acids research. Supplement (2001). 3 edn, pp. 293-294.
Ando T, Tanaka T, Kikuchi Y. Bacterial ribonuclease P reaction is affected by substrate shape and magnesium ion concentration. In Nucleic acids research. Supplement (2001). 3 ed. 2003. p. 293-294
Ando, Tomoaki ; Tanaka, Terumichi ; Kikuchi, Yo. / Bacterial ribonuclease P reaction is affected by substrate shape and magnesium ion concentration. Nucleic acids research. Supplement (2001). 3. ed. 2003. pp. 293-294
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