Basic Fibroblast Growth Factor-Like Substance Associates with DNA in Germ Cell Nuclei

Takayuki Kamei, Katsushi Suzuki, Kunio Shiota, Tomoya Ogawa, Michio Takahashi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Our previous work has demonstrated that immunoreactive basic fibroblast growth factor (bFGF)-like substance presents in germ cell nuclei of adult rat testis. In the present study, interactions of bFGF-like substance with a sub-nuclear material were investigated. When nuclear extract from adult rat germ cell was analysed by heparin-Sepharose affinity chromatography and Western blot, germ cell nuclei contained 2 forms of bFGF-like substances with molecular weights of 16.5 and 24.5 kDa. The immunoreactivity for bFGF-like substance of germ cell nuclei decreased after treatment with either micrococcal nuclease or DNase I. Furthermore, South-Western blots analysis revealed these 2 forms of bFGF-like substance bind with high affinity to the exogenous salmon testis DNA. Thus, these results suggest that the bFGF-like substances are associated with DNA in the germ cel.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalJournal of Reproduction and Development
Volume39
Issue number1
DOIs
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

fibroblast growth factor 2
cell nucleus
germ cells
DNA
testes
Western blotting
micrococcal nuclease
deoxyribonuclease I
rats
heparin
affinity chromatography
agarose
salmon
molecular weight
extracts

Keywords

  • Basic fibroblast growth factor
  • DNA-binding
  • Nuclear localization
  • Testis

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Basic Fibroblast Growth Factor-Like Substance Associates with DNA in Germ Cell Nuclei. / Kamei, Takayuki; Suzuki, Katsushi; Shiota, Kunio; Ogawa, Tomoya; Takahashi, Michio.

In: Journal of Reproduction and Development, Vol. 39, No. 1, 1993, p. 25-31.

Research output: Contribution to journalArticle

Kamei, Takayuki ; Suzuki, Katsushi ; Shiota, Kunio ; Ogawa, Tomoya ; Takahashi, Michio. / Basic Fibroblast Growth Factor-Like Substance Associates with DNA in Germ Cell Nuclei. In: Journal of Reproduction and Development. 1993 ; Vol. 39, No. 1. pp. 25-31.
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