Bifidobacterium kashiwanohense sp. nov., isolated from healthy infant faeces

Hidetoshi Morita, Akiyo Nakano, Hiromi Onoda, Hidehiro Toh, Kenshiro Oshima, Hideto Takami, Masaru Murakami, Shinji Fukuda, Tatsuya Takizawa, Tomomi Kuwahara, Hiroshi Ohno, Soichi Tanabe, Masahira Hattori

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Strains HM2-1 and HM2-2 T were isolated from the faeces of a healthy infant and were characterized by determining their phenotypic and biochemical features and phylogenetic positions based on partial 16S rRNA gene sequence analysis. They were Gram-positive, obligately anaerobic, non-spore-forming, non-gas-producing, and catalase-negative non-motile rods. They did not grow at 15 or 45 °C in anaerobic bacterial culture medium, and their DNA G+C content was in the range 56-59 mol%. In enzyme activity tests, strains HM2-1 and HM2-2 T were positive for α/β-galactosidases and α/β-glucosidases but negative for β-glucuronidase and cystine arylamidase. An analysis of the cell-wall composition of strains HM2-1 and HM2-2 T revealed the presence of glutamic acid, alanine and lysine. The presence of fructose-6-phosphate phosphoketolase shows that isolates HM2-1 and HM2-2 T are members of the genus Bifidobacterium. These two isolates belong to the same species of the genus Bifidobacterium. Strain HM2-2T was found to be related to Bifidobacterium catenulatum JCM 1194 T (97.4% 16S rRNA gene sequence identity: 1480/1520 bp), Bifidobacterium pseudocatenulatum JCM 1200T (97.2%: 1472/1514 bp), Bifidobacterium dentium ATCC 27534 T (96.7%: 1459/1509 bp) and Bifidobacterium angulatum ATCC 27535 T (96.5%: 1462/1515 bp). The predominant cellular fatty acids of strains HM2-1 and HM2-2 T were 16: 0 and 18: 1ω9c, with proportions greater than 18% of the total. Phylogenetic analyses involving phenotypic characterization, DNA-DNA hybridization and partial 16S rRNA gene sequencing proves that the strains represent a novel species of the genus Bifidobacterium, for which the name Bifidobacterium kashiwanohense sp. nov. is proposed. The type strain is HM2-2 T (=JCM 15439 T =DSM 21854 T).

Original languageEnglish
Pages (from-to)2610-2615
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume61
Issue number11
DOIs
Publication statusPublished - 2011 Nov
Externally publishedYes

Fingerprint

Bifidobacterium
infants
Feces
feces
DNA
gene
new species
phylogenetics
rRNA Genes
ribosomal RNA
enzyme activity
fatty acid
Bifidobacterium angulatum
Bifidobacterium dentium
Bifidobacterium catenulatum
phosphate
Bifidobacterium pseudocatenulatum
phosphoketolase
Cystinyl Aminopeptidase
Galactosidases

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Bifidobacterium kashiwanohense sp. nov., isolated from healthy infant faeces. / Morita, Hidetoshi; Nakano, Akiyo; Onoda, Hiromi; Toh, Hidehiro; Oshima, Kenshiro; Takami, Hideto; Murakami, Masaru; Fukuda, Shinji; Takizawa, Tatsuya; Kuwahara, Tomomi; Ohno, Hiroshi; Tanabe, Soichi; Hattori, Masahira.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 61, No. 11, 11.2011, p. 2610-2615.

Research output: Contribution to journalArticle

Morita, H, Nakano, A, Onoda, H, Toh, H, Oshima, K, Takami, H, Murakami, M, Fukuda, S, Takizawa, T, Kuwahara, T, Ohno, H, Tanabe, S & Hattori, M 2011, 'Bifidobacterium kashiwanohense sp. nov., isolated from healthy infant faeces', International Journal of Systematic and Evolutionary Microbiology, vol. 61, no. 11, pp. 2610-2615. https://doi.org/10.1099/ijs.0.024521-0
Morita, Hidetoshi ; Nakano, Akiyo ; Onoda, Hiromi ; Toh, Hidehiro ; Oshima, Kenshiro ; Takami, Hideto ; Murakami, Masaru ; Fukuda, Shinji ; Takizawa, Tatsuya ; Kuwahara, Tomomi ; Ohno, Hiroshi ; Tanabe, Soichi ; Hattori, Masahira. / Bifidobacterium kashiwanohense sp. nov., isolated from healthy infant faeces. In: International Journal of Systematic and Evolutionary Microbiology. 2011 ; Vol. 61, No. 11. pp. 2610-2615.
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AU - Murakami, Masaru

AU - Fukuda, Shinji

AU - Takizawa, Tatsuya

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N2 - Strains HM2-1 and HM2-2 T were isolated from the faeces of a healthy infant and were characterized by determining their phenotypic and biochemical features and phylogenetic positions based on partial 16S rRNA gene sequence analysis. They were Gram-positive, obligately anaerobic, non-spore-forming, non-gas-producing, and catalase-negative non-motile rods. They did not grow at 15 or 45 °C in anaerobic bacterial culture medium, and their DNA G+C content was in the range 56-59 mol%. In enzyme activity tests, strains HM2-1 and HM2-2 T were positive for α/β-galactosidases and α/β-glucosidases but negative for β-glucuronidase and cystine arylamidase. An analysis of the cell-wall composition of strains HM2-1 and HM2-2 T revealed the presence of glutamic acid, alanine and lysine. The presence of fructose-6-phosphate phosphoketolase shows that isolates HM2-1 and HM2-2 T are members of the genus Bifidobacterium. These two isolates belong to the same species of the genus Bifidobacterium. Strain HM2-2T was found to be related to Bifidobacterium catenulatum JCM 1194 T (97.4% 16S rRNA gene sequence identity: 1480/1520 bp), Bifidobacterium pseudocatenulatum JCM 1200T (97.2%: 1472/1514 bp), Bifidobacterium dentium ATCC 27534 T (96.7%: 1459/1509 bp) and Bifidobacterium angulatum ATCC 27535 T (96.5%: 1462/1515 bp). The predominant cellular fatty acids of strains HM2-1 and HM2-2 T were 16: 0 and 18: 1ω9c, with proportions greater than 18% of the total. Phylogenetic analyses involving phenotypic characterization, DNA-DNA hybridization and partial 16S rRNA gene sequencing proves that the strains represent a novel species of the genus Bifidobacterium, for which the name Bifidobacterium kashiwanohense sp. nov. is proposed. The type strain is HM2-2 T (=JCM 15439 T =DSM 21854 T).

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