Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole

Junko Y. Toshima, Show Nishinoaki, Yoshifumi Sato, Wataru Yamamoto, Daiki Furukawa, Daria Elisabeth Siekhaus, Akira Sawaguchi, Jiro Toshima

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The yeast Rab5 homologue, Vps21p, is known to be involved both in the vacuolar protein sorting (VPS) pathway from the trans-Golgi network to the vacuole, and in the endocytic pathway from the plasma membrane to the vacuole. However, the intracellular location at which these two pathways converge remains unclear. In addition, the endocytic pathway is not completely blocked in yeast cells lacking all Rab5 genes, suggesting the existence of an unidentified route that bypasses the Rab5-dependent endocytic pathway. Here we show that convergence of the endocytic and VPS pathways occurs upstream of the requirement for Vps21p in these pathways. We also identify a previously unidentified endocytic pathway mediated by the AP-3 complex. Importantly, the AP-3-mediated pathway appears mostly intact in Rab5-disrupted cells, and thus works as an alternative route to the vacuole/lysosome. We propose that the endocytic traffic branches into two routes to reach the vacuole: a Rab5-dependent VPS pathway and a Rab5-independent AP-3-mediated pathway.

Original languageEnglish
Article number3498
JournalNature Communications
Volume5
DOIs
Publication statusPublished - 2014 Mar 25

Fingerprint

Vacuoles
classifying
Sorting
Protein Transport
yeast
routes
proteins
Yeast
lysosomes
Proteins
Yeasts
bypasses
Cell membranes
trans-Golgi Network
genes
upstream
traffic
Genes
Lysosomes
Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Toshima, J. Y., Nishinoaki, S., Sato, Y., Yamamoto, W., Furukawa, D., Siekhaus, D. E., ... Toshima, J. (2014). Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole. Nature Communications, 5, [3498]. https://doi.org/10.1038/ncomms4498

Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole. / Toshima, Junko Y.; Nishinoaki, Show; Sato, Yoshifumi; Yamamoto, Wataru; Furukawa, Daiki; Siekhaus, Daria Elisabeth; Sawaguchi, Akira; Toshima, Jiro.

In: Nature Communications, Vol. 5, 3498, 25.03.2014.

Research output: Contribution to journalArticle

Toshima, JY, Nishinoaki, S, Sato, Y, Yamamoto, W, Furukawa, D, Siekhaus, DE, Sawaguchi, A & Toshima, J 2014, 'Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole', Nature Communications, vol. 5, 3498. https://doi.org/10.1038/ncomms4498
Toshima JY, Nishinoaki S, Sato Y, Yamamoto W, Furukawa D, Siekhaus DE et al. Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole. Nature Communications. 2014 Mar 25;5. 3498. https://doi.org/10.1038/ncomms4498
Toshima, Junko Y. ; Nishinoaki, Show ; Sato, Yoshifumi ; Yamamoto, Wataru ; Furukawa, Daiki ; Siekhaus, Daria Elisabeth ; Sawaguchi, Akira ; Toshima, Jiro. / Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole. In: Nature Communications. 2014 ; Vol. 5.
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