Big softer hole on living cells: elasticity imaging with AFM

Kazushige Kawabata, Hisashi Haga, Takahiro Nitta, Yuusuke Endo, Masafumi Nagayama, Etsuro Ito, Takashi Sambongi

Research output: Contribution to journalConference article

1 Citation (Scopus)

Abstract

We have focused on effects of local mechanical properties of the cell on cell motion. By using atomic force microscopy, we measured spatial distribution of local elastic modulus on mouse fibroblasts (NIH3T3), which is living in a physiological condition. In order to examine validity of AFM elastic measurements, we measured local elastic modulus of gels as elastic reference materials. The results obtained with AFM were compared with values obtained by tensile creep method. It is veryfied that these values are proportional each other. The AFM experiments on living cells revealed that center area of cell surface is about 10 times softer than the surroundings and looks like a big softer hole in the elasticity image. We fixed the cell just after the AFM measurements and carried out immunofluorescence observation for cytoskeletal filaments of actin filaments, microtubules and intermediate filaments. A comparison between distribution of local elasticity and cytoskeletones indicates that harder area on the cell results mainly from concentration of actin filaments. However, we found that some areas like the big softer hole do not correspond to distribution of actin filaments.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3922
Publication statusPublished - 2000 Jan 1
Externally publishedYes
EventScanning and Force Microscopies for Biomedical Applications II - San Jose, CA, USA
Duration: 2000 Jan 232000 Jan 24

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Kawabata, K., Haga, H., Nitta, T., Endo, Y., Nagayama, M., Ito, E., & Sambongi, T. (2000). Big softer hole on living cells: elasticity imaging with AFM. Proceedings of SPIE - The International Society for Optical Engineering, 3922, 91-98.