Binding of lysozyme onto a cation-exchange microporous membrane containing tentacle-type grafted polymer branches

Satoshi Tsuneda, H. Shinano, K. Saito, S. Furusaki, T. Sugo

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Ion-exchange adsorption of lysozyme to the sulfonic acid (SO3H) group on polymer chains grafted onto microporous polyethylene hollow-fiber membranes was examined. The lysozyme solution was forced to permeate across the hollow fiber. Diversely anchored SO3H groups, i.e., SP and SS groups, were introduced into the membrane by reaction of the glycidyl methacrylate-grafted membrane with propanesultone and sodium sulfite, respectively. The resulting SP and SS group-containing membranes, designated as SP-T and SS-T fibers, respectively, had 95 and 77% water flux of the original membrane, respectively. The binding capacity of lysozyme as a function of the SO3H group density was compared between the SP-T and SS-T fibers from measurement of the ion-exchange breakthrough curves during the permeation of lysozyme solution across the SP-T and SS-T fibers. The binding capacity of lysozyme to the SP-T fiber remained constant, independent of the SP group density, whereas that to the SS-T fiber increased linearly with increasing SS group density. This difference was explained by means of a model whereby lysozyme adheres onto the SP group-containing grafted polymer branches, while the SS group-containing grafted polymer branches hold lysozyme in a tentacle-like manner.

Original languageEnglish
Pages (from-to)76-81
Number of pages6
JournalBiotechnology Progress
Volume10
Issue number1
Publication statusPublished - 1994 Jan
Externally publishedYes

Fingerprint

cation exchange
Muramidase
lysozyme
Cations
polymers
Polymers
Membranes
Ion Exchange
binding capacity
ion exchange
sodium sulfite
Sulfonic Acids
permeates
sulfonic acid
Polyethylene
polyethylene
Adsorption
adsorption
Water

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Microbiology

Cite this

Binding of lysozyme onto a cation-exchange microporous membrane containing tentacle-type grafted polymer branches. / Tsuneda, Satoshi; Shinano, H.; Saito, K.; Furusaki, S.; Sugo, T.

In: Biotechnology Progress, Vol. 10, No. 1, 01.1994, p. 76-81.

Research output: Contribution to journalArticle

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