Biocatalytic synthesis of dihydroxynaphthoic acids by cytochrome P450 CYP199A2

Toshiki Furuya, Kuniki Kino

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    CYP199A2, a bacterial P450 monooxygenase from Rhodopseudomonas palustris, was found to exhibit oxidation activity towards three hydroxynaphthoic acids. Whole cells of the recombinant Escherichia coli strain expressing CYP199A2 efficiently catalyzed the regioselective oxidation of 1-, 3-, and 6-hydroxy-2-naphthoic acids to produce 1,7-, 3,7-, and 6,7-dihydroxynaphthoic acid respectively. These results suggest that CYP199A2 might be a useful oxidation biocatalyst for the synthesis of dihydroxynaphthoic acids.

    Original languageEnglish
    Pages (from-to)2797-2799
    Number of pages3
    JournalBioscience, Biotechnology and Biochemistry
    Volume73
    Issue number12
    DOIs
    Publication statusPublished - 2009

    Fingerprint

    Cytochrome P-450 Enzyme System
    Oxidation
    Acids
    Rhodopseudomonas
    Mixed Function Oxygenases
    Escherichia coli
    Enzymes
    2-naphthoic acid
    3-hydroxy-2-naphthoic acid

    Keywords

    • CYP199A2
    • Cytochrome p450
    • Hydroxylation
    • Naphthoic acid
    • Oxidation

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry
    • Molecular Biology
    • Applied Microbiology and Biotechnology
    • Analytical Chemistry
    • Organic Chemistry
    • Medicine(all)

    Cite this

    Biocatalytic synthesis of dihydroxynaphthoic acids by cytochrome P450 CYP199A2. / Furuya, Toshiki; Kino, Kuniki.

    In: Bioscience, Biotechnology and Biochemistry, Vol. 73, No. 12, 2009, p. 2797-2799.

    Research output: Contribution to journalArticle

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