Biochemical analysis of the human EVL domains in homologous recombination

Motoki Takaku, Shinichi MacHida, Shugo Nakayama, Daisuke Takahashi, Hitoshi Kurumizaka

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    EVL is a member of the ENA/VASP family, which is involved in actin-remodeling processes. Previously, we reported that human EVL directly interacts with RAD51, which is an essential protein in the homologous recombinational repair of DNA double-strand breaks, and stimulates RAD51-mediated recombination reactions in vitro. To identify the EVL domain required for the recombination function, we purified the EVL fragments EVL(1-221) and EVL(222-418), which contain the EVH1 and Pro-rich domains and the EVH2 domain, respectively. We found that EVL(222-418) possesses DNA-binding and RAD51-binding activities, and also stimulates RAD51-mediated homologous pairing. In contrast, EVL(1-221) did not exhibit any of these activities. Therefore, the EVH2 domain, which is highly conserved among the ENA/VASP family proteins, may be responsible for the recombination function of EVL.

    Original languageEnglish
    Pages (from-to)5841-5848
    Number of pages8
    JournalFEBS Journal
    Volume276
    Issue number20
    DOIs
    Publication statusPublished - 2009 Oct

    Fingerprint

    Homologous Recombination
    Genetic Recombination
    Recombinational DNA Repair
    DNA
    Actins
    Proteins
    Repair

    Keywords

    • DSB
    • ENA/VASP
    • EVL
    • Homologous recombination
    • RAD51

    ASJC Scopus subject areas

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Cite this

    Biochemical analysis of the human EVL domains in homologous recombination. / Takaku, Motoki; MacHida, Shinichi; Nakayama, Shugo; Takahashi, Daisuke; Kurumizaka, Hitoshi.

    In: FEBS Journal, Vol. 276, No. 20, 10.2009, p. 5841-5848.

    Research output: Contribution to journalArticle

    Takaku, Motoki ; MacHida, Shinichi ; Nakayama, Shugo ; Takahashi, Daisuke ; Kurumizaka, Hitoshi. / Biochemical analysis of the human EVL domains in homologous recombination. In: FEBS Journal. 2009 ; Vol. 276, No. 20. pp. 5841-5848.
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