Biodesulfurization of dibenzothiophene and its derivatives through the selective cleavage of carbon-sulfur bonds by a moderately thermophilic bacterium Bacillus subtilis WU-S2B

Kotaro Kirimura, Toshiki Furuya, Yasuhiro Nishii, Yoshitaka Ishii, Kuniki Kino, Shoji Usami

    Research output: Contribution to journalArticle

    115 Citations (Scopus)

    Abstract

    Heterocyclic organosulfur compounds such as dibenzothiophene (DBT) in petroleum cannot be completely removed by hydrodesulfurization using chemical catalysts. A moderately thermophilic bacterium Bacillus subtilis WU-S2B, which could desulfurize DBT at 50°C through the selective cleavage of carbon-sulfur (C-S) bonds, was newly isolated. At 50°C, growing cells of WU-S2B could degrade 0.54 mM DBT within 120 h to produce 2-hydroxybiphenyl, and the resting cells could also degrade 0.81 mM DBT within 12 h. The DBT-desulfurizing ability of WU-S2B is high over a wide temperature range from 30 to 50°C, and highest at 50°C for both the growing and resting cells, and this is an extremely advantageous property for the practical biodesulfurization. In addition, WU-S2B could also desulfurize DBT derivatives such as 2,8-dimethyIDBT, 4,6-dimethyIDBT and 3,4-benzoDBT. Therefore, B. subtilis WU-S2B is considered to have more beneficial properties than other desulfurizing bacteria such as Rhodococcus strains previously reported, particularly from the viewpoint of its capacity for thermophilic desulfurizatiou through the C-S bond cleavage.

    Original languageEnglish
    Pages (from-to)262-266
    Number of pages5
    JournalJournal of Bioscience and Bioengineering
    Volume91
    Issue number3
    DOIs
    Publication statusPublished - 2001

    Fingerprint

    Bacilli
    Desulfurization
    Bacillus subtilis
    Sulfur
    Bacteria
    Carbon
    Derivatives
    Hydrodesulfurization
    Crude oil
    Catalysts
    Heterocyclic Compounds
    Rhodococcus
    Petroleum
    Temperature
    dibenzothiophene

    Keywords

    • Bacillus subtilis
    • Carbon-sulfur bond cleavage
    • Desulfurization
    • Dibenzothiophene
    • Petroleum

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering

    Cite this

    @article{5a98389890e14b4293791738b5806bdd,
    title = "Biodesulfurization of dibenzothiophene and its derivatives through the selective cleavage of carbon-sulfur bonds by a moderately thermophilic bacterium Bacillus subtilis WU-S2B",
    abstract = "Heterocyclic organosulfur compounds such as dibenzothiophene (DBT) in petroleum cannot be completely removed by hydrodesulfurization using chemical catalysts. A moderately thermophilic bacterium Bacillus subtilis WU-S2B, which could desulfurize DBT at 50°C through the selective cleavage of carbon-sulfur (C-S) bonds, was newly isolated. At 50°C, growing cells of WU-S2B could degrade 0.54 mM DBT within 120 h to produce 2-hydroxybiphenyl, and the resting cells could also degrade 0.81 mM DBT within 12 h. The DBT-desulfurizing ability of WU-S2B is high over a wide temperature range from 30 to 50°C, and highest at 50°C for both the growing and resting cells, and this is an extremely advantageous property for the practical biodesulfurization. In addition, WU-S2B could also desulfurize DBT derivatives such as 2,8-dimethyIDBT, 4,6-dimethyIDBT and 3,4-benzoDBT. Therefore, B. subtilis WU-S2B is considered to have more beneficial properties than other desulfurizing bacteria such as Rhodococcus strains previously reported, particularly from the viewpoint of its capacity for thermophilic desulfurizatiou through the C-S bond cleavage.",
    keywords = "Bacillus subtilis, Carbon-sulfur bond cleavage, Desulfurization, Dibenzothiophene, Petroleum",
    author = "Kotaro Kirimura and Toshiki Furuya and Yasuhiro Nishii and Yoshitaka Ishii and Kuniki Kino and Shoji Usami",
    year = "2001",
    doi = "10.1016/S1389-1723(01)80131-6",
    language = "English",
    volume = "91",
    pages = "262--266",
    journal = "Journal of Bioscience and Bioengineering",
    issn = "1389-1723",
    publisher = "Elsevier",
    number = "3",

    }

    TY - JOUR

    T1 - Biodesulfurization of dibenzothiophene and its derivatives through the selective cleavage of carbon-sulfur bonds by a moderately thermophilic bacterium Bacillus subtilis WU-S2B

    AU - Kirimura, Kotaro

    AU - Furuya, Toshiki

    AU - Nishii, Yasuhiro

    AU - Ishii, Yoshitaka

    AU - Kino, Kuniki

    AU - Usami, Shoji

    PY - 2001

    Y1 - 2001

    N2 - Heterocyclic organosulfur compounds such as dibenzothiophene (DBT) in petroleum cannot be completely removed by hydrodesulfurization using chemical catalysts. A moderately thermophilic bacterium Bacillus subtilis WU-S2B, which could desulfurize DBT at 50°C through the selective cleavage of carbon-sulfur (C-S) bonds, was newly isolated. At 50°C, growing cells of WU-S2B could degrade 0.54 mM DBT within 120 h to produce 2-hydroxybiphenyl, and the resting cells could also degrade 0.81 mM DBT within 12 h. The DBT-desulfurizing ability of WU-S2B is high over a wide temperature range from 30 to 50°C, and highest at 50°C for both the growing and resting cells, and this is an extremely advantageous property for the practical biodesulfurization. In addition, WU-S2B could also desulfurize DBT derivatives such as 2,8-dimethyIDBT, 4,6-dimethyIDBT and 3,4-benzoDBT. Therefore, B. subtilis WU-S2B is considered to have more beneficial properties than other desulfurizing bacteria such as Rhodococcus strains previously reported, particularly from the viewpoint of its capacity for thermophilic desulfurizatiou through the C-S bond cleavage.

    AB - Heterocyclic organosulfur compounds such as dibenzothiophene (DBT) in petroleum cannot be completely removed by hydrodesulfurization using chemical catalysts. A moderately thermophilic bacterium Bacillus subtilis WU-S2B, which could desulfurize DBT at 50°C through the selective cleavage of carbon-sulfur (C-S) bonds, was newly isolated. At 50°C, growing cells of WU-S2B could degrade 0.54 mM DBT within 120 h to produce 2-hydroxybiphenyl, and the resting cells could also degrade 0.81 mM DBT within 12 h. The DBT-desulfurizing ability of WU-S2B is high over a wide temperature range from 30 to 50°C, and highest at 50°C for both the growing and resting cells, and this is an extremely advantageous property for the practical biodesulfurization. In addition, WU-S2B could also desulfurize DBT derivatives such as 2,8-dimethyIDBT, 4,6-dimethyIDBT and 3,4-benzoDBT. Therefore, B. subtilis WU-S2B is considered to have more beneficial properties than other desulfurizing bacteria such as Rhodococcus strains previously reported, particularly from the viewpoint of its capacity for thermophilic desulfurizatiou through the C-S bond cleavage.

    KW - Bacillus subtilis

    KW - Carbon-sulfur bond cleavage

    KW - Desulfurization

    KW - Dibenzothiophene

    KW - Petroleum

    UR - http://www.scopus.com/inward/record.url?scp=0035055057&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0035055057&partnerID=8YFLogxK

    U2 - 10.1016/S1389-1723(01)80131-6

    DO - 10.1016/S1389-1723(01)80131-6

    M3 - Article

    C2 - 16232986

    AN - SCOPUS:0035055057

    VL - 91

    SP - 262

    EP - 266

    JO - Journal of Bioscience and Bioengineering

    JF - Journal of Bioscience and Bioengineering

    SN - 1389-1723

    IS - 3

    ER -