Bioengineering of bacterial magnetic particles and its application to estrogen receptor-ligand binding assay

Tomoko Yoshino, Chihiro Kaji, Tadashi Matsunaga

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Magnetic particles are used for various biomedical applications because they are easy to both handle and separate from biological samples. Nano-sized bacterial magnetic particles (BacMPs) that display the human estrogen receptor ligand binding domain (ERLBD) on their surfaces were successfully produced by the magnetotactic bacterium, Magnetospirillum inagneticum AMB-1. A receptor assay for endocrine-disrupting chemicals using ERLBD- displaying BacMPs was developed. A BacMP membrane-specific protein, Mmsl6 or Mmsl3, was used as an anchor protein to localize the ERLBD on the surfaces of BacMPs. ERLBD- BacMP complexes were assayed for competitive binding of alkaline phosphatase- conjugated 17(3-estradiol (ALP-E2). Inhibition curve of ALP-E2 to the powerful antagonist, tamoxifen was generated by measuring decreases in luminescence intensity that resulted from the enzymatic reaction of alkaline phosphatase. The overall simplicity of this receptor-binding assay results in a method that can be easily adapted to a high-throughput format.

Original languageEnglish
Title of host publicationFrom Biological Materials to Biomimetic Material Synthesis
Pages13-18
Number of pages6
Volume1094
Publication statusPublished - 2008
Externally publishedYes
Event2008 MRS Spring Meeting - San Francisco, CA
Duration: 2008 Mar 242008 Mar 28

Other

Other2008 MRS Spring Meeting
CitySan Francisco, CA
Period08/3/2408/3/28

Fingerprint

bioengineering
estrogens
Estrogen Receptors
Assays
Ligands
ligands
Phosphatases
Alkaline Phosphatase
phosphatases
Proteins
Endocrine Disruptors
Tamoxifen
Anchors
proteins
Luminescence
disrupting
Estradiol
Bacteria
Throughput
Membranes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Yoshino, T., Kaji, C., & Matsunaga, T. (2008). Bioengineering of bacterial magnetic particles and its application to estrogen receptor-ligand binding assay. In From Biological Materials to Biomimetic Material Synthesis (Vol. 1094, pp. 13-18)

Bioengineering of bacterial magnetic particles and its application to estrogen receptor-ligand binding assay. / Yoshino, Tomoko; Kaji, Chihiro; Matsunaga, Tadashi.

From Biological Materials to Biomimetic Material Synthesis. Vol. 1094 2008. p. 13-18.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yoshino, T, Kaji, C & Matsunaga, T 2008, Bioengineering of bacterial magnetic particles and its application to estrogen receptor-ligand binding assay. in From Biological Materials to Biomimetic Material Synthesis. vol. 1094, pp. 13-18, 2008 MRS Spring Meeting, San Francisco, CA, 08/3/24.
Yoshino T, Kaji C, Matsunaga T. Bioengineering of bacterial magnetic particles and its application to estrogen receptor-ligand binding assay. In From Biological Materials to Biomimetic Material Synthesis. Vol. 1094. 2008. p. 13-18
Yoshino, Tomoko ; Kaji, Chihiro ; Matsunaga, Tadashi. / Bioengineering of bacterial magnetic particles and its application to estrogen receptor-ligand binding assay. From Biological Materials to Biomimetic Material Synthesis. Vol. 1094 2008. pp. 13-18
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