Biohybrid Actuators Based on Skeletal Muscle-Powered Microgrooved Ultrathin Films Consisting of Poly(styrene- block-butadiene- block-styrene)

Arihiro Hasebe, Yoshitaka Suematsu, Shinji Takeoka, Tommaso Mazzocchi, Lorenzo Vannozzi, Leonardo Ricotti, Toshinori Fujie

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This paper describes a biohybrid actuator consisting of a microgrooved thin film, powered by contractile, aligned skeletal muscle cells. The system was made of a thermoplastic elastomer [SBS, poly(styrene-block-butadiene-block-styrene)]. We prepared SBS thin films with different thicknesses (0.5-11.7 μm) and Young's moduli (46.7-68.6 MPa) to vary their flexural rigidity. The microgrooves on the SBS thin film resembled the microstructure of the extracellular matrix of muscle and facilitated the alignment and differentiation of skeletal muscle cells. Electrical stimulation was applied to self-standing biohybrid thin films to trigger their contraction, enabled by the low flexural rigidity of the SBS thin film. Finite element model simulations were also examined to predict their contractile behavior. We achieved the prediction of displacements, which were rather close to the actual values of the SBS thin film: the discrepancy was <5% on the X axis. These results pave the way for in silico prediction of the contractile capabilities of elastomeric thin films. This study highlights the potential of microgrooved SBS thin films as ultraflexible platforms for biohybrid machines.

Original languageEnglish
JournalACS Biomaterials Science and Engineering
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Styrene
Ultrathin films
Butadiene
Muscle
Actuators
Thin films
Rigidity
1,3-butadiene
Cells
Thermoplastic elastomers
Elastic moduli

Keywords

  • biohybrid actuator
  • flexural rigidity
  • microgrooves
  • organic machine
  • polymeric thin films
  • SBS
  • skeletal muscle

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Biohybrid Actuators Based on Skeletal Muscle-Powered Microgrooved Ultrathin Films Consisting of Poly(styrene- block-butadiene- block-styrene). / Hasebe, Arihiro; Suematsu, Yoshitaka; Takeoka, Shinji; Mazzocchi, Tommaso; Vannozzi, Lorenzo; Ricotti, Leonardo; Fujie, Toshinori.

In: ACS Biomaterials Science and Engineering, 01.01.2019.

Research output: Contribution to journalArticle

Hasebe, Arihiro ; Suematsu, Yoshitaka ; Takeoka, Shinji ; Mazzocchi, Tommaso ; Vannozzi, Lorenzo ; Ricotti, Leonardo ; Fujie, Toshinori. / Biohybrid Actuators Based on Skeletal Muscle-Powered Microgrooved Ultrathin Films Consisting of Poly(styrene- block-butadiene- block-styrene). In: ACS Biomaterials Science and Engineering. 2019.
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