Bioimaging with two-photon-induced luminescence from triangular nanoplates and nanoparticle aggregates of gold

Yuqiang Jiang, Noriko Nishizawa Horimoto, Kohei Imura, Hiromi Okamoto, Ko Matsui, Ryuichi Shigemoto

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The living cell imaging using a two-photon microscope using gold nanoplates and nanoparticle aggregates was demonstrated. The dimensions of the nanoplates were determined through scanning electron microscopy (SEM) and atomic force microscopy. The height of a 100 nm base-length nanotriangle was around 10 nm, while the height of 300 nm base-length nanotriangle was around 12 nm. A spectrophotometer was also used to determine the extinction spectra of gold nanoparticle colloids. Two-photon-induced photoluminescence (TPIPL) under far-field excitation was tested for gold nanoplates on a glass substrate using two-photon laser scanning microscopy (TPLSM). It was observed that living-cell microscopic imaging can be carried out with TPIPL from gold nanoplates and aggregated nanosphere. This method provided a platform for developing tools for biological and biomedical studies.

Original languageEnglish
Pages (from-to)2309-2313
Number of pages5
JournalAdvanced Materials
Volume21
Issue number22
DOIs
Publication statusPublished - 2009 Jun 12
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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