Bioimaging with two-photon-induced luminescence from triangular nanoplates and nanoparticle aggregates of gold

Yuqiang Jiang, Noriko Nishizawa Horimoto, Kohei Imura, Hiromi Okamoto, Ko Matsui, Ryuichi Shigemoto

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The living cell imaging using a two-photon microscope using gold nanoplates and nanoparticle aggregates was demonstrated. The dimensions of the nanoplates were determined through scanning electron microscopy (SEM) and atomic force microscopy. The height of a 100 nm base-length nanotriangle was around 10 nm, while the height of 300 nm base-length nanotriangle was around 12 nm. A spectrophotometer was also used to determine the extinction spectra of gold nanoparticle colloids. Two-photon-induced photoluminescence (TPIPL) under far-field excitation was tested for gold nanoplates on a glass substrate using two-photon laser scanning microscopy (TPLSM). It was observed that living-cell microscopic imaging can be carried out with TPIPL from gold nanoplates and aggregated nanosphere. This method provided a platform for developing tools for biological and biomedical studies.

Original languageEnglish
Pages (from-to)2309-2313
Number of pages5
JournalAdvanced Materials
Volume21
Issue number22
DOIs
Publication statusPublished - 2009 Jun 12
Externally publishedYes

Fingerprint

Gold
Luminescence
Photons
Nanoparticles
Photoluminescence
Imaging techniques
Light extinction
Nanospheres
Spectrophotometers
Colloids
Atomic force microscopy
Microscopic examination
Microscopes
Cells
Scanning
Glass
Scanning electron microscopy
Lasers
Substrates

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Bioimaging with two-photon-induced luminescence from triangular nanoplates and nanoparticle aggregates of gold. / Jiang, Yuqiang; Horimoto, Noriko Nishizawa; Imura, Kohei; Okamoto, Hiromi; Matsui, Ko; Shigemoto, Ryuichi.

In: Advanced Materials, Vol. 21, No. 22, 12.06.2009, p. 2309-2313.

Research output: Contribution to journalArticle

Jiang, Yuqiang ; Horimoto, Noriko Nishizawa ; Imura, Kohei ; Okamoto, Hiromi ; Matsui, Ko ; Shigemoto, Ryuichi. / Bioimaging with two-photon-induced luminescence from triangular nanoplates and nanoparticle aggregates of gold. In: Advanced Materials. 2009 ; Vol. 21, No. 22. pp. 2309-2313.
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