Biological determination of Ag(I) ion and arginine by using the composite film of electroinactive polypyrrole and polyion complex

Shinichi Komaba, Yusuke Fujino, Tomoka Matsuda, Tetsuya Osaka, Ikuo Satoh

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28 Citations (Scopus)


The urea biosensor based on the combination of electropolymerized insulating polypyrrole (PPy) with urease was used for the determination of Ag(I) ion and arginine. The inhibition ratio of urease with Ag(I) ion was evaluated from the urea response of the sensor. This method enabled the detection of 1.0 × 10-7 to 1.0 × 10-4 mol dm-3 of Ag(I) ion. With the addition of EDTA as a masking agent for interfering heavy metal ions, the selectivity for Ag(I) ion was improved. Furthermore, arginine detection was carried out with the PPy electrode using bienzymatic combination of urease and arginase. The calibration curve for arginine was obtained in the concentration range of 1.0 × 10-5 to 1.0 × 10-3 mol dm-3 with a sensitivity of 50 mV decade-1.

Original languageEnglish
Pages (from-to)78-83
Number of pages6
JournalSensors and Actuators, B: Chemical
Issue number1-2
Publication statusPublished - 1998 Sep 15



  • Ag(I) ion
  • Arginine
  • Biosensor
  • Polypyrrole
  • Urease

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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