Biological determination of Ag(I) ion and arginine by using the composite film of electroinactive polypyrrole and polyion complex

Shinichi Komaba, Yusuke Fujino, Tomoka Matsuda, Tetsuya Osaka, Ikuo Satoh

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The urea biosensor based on the combination of electropolymerized insulating polypyrrole (PPy) with urease was used for the determination of Ag(I) ion and arginine. The inhibition ratio of urease with Ag(I) ion was evaluated from the urea response of the sensor. This method enabled the detection of 1.0×10-7 to 1.0×10-4 mol dm-3 of Ag(I) ion. With the addition of EDTA as a masking agent for interfering heavy metal ions, the selectivity for Ag(I) ion was improved. Furthermore, arginine detection was carried out with the PPy electrode using bienzymatic combination of urease and arginase. The calibration curve for arginine was obtained in the concentration range of 1.0×10-5 to 1.0×10-3 mol dm-3 with a sensitivity of 50 mV decade-1.

Original languageEnglish
Pages (from-to)78-83
Number of pages6
JournalSensors and Actuators, B: Chemical
VolumeB52
Issue number1-2
Publication statusPublished - 1998 Jan 1

Fingerprint

Arginine
polypyrroles
Polypyrroles
Composite films
Urease
Ions
composite materials
ureas
Urea
ions
Arginase
ethylenediaminetetraacetic acids
Ethylenediaminetetraacetic acid
heavy metals
masking
Heavy Metals
Heavy ions
bioinstrumentation
Biosensors
Edetic Acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry
  • Electrical and Electronic Engineering

Cite this

Biological determination of Ag(I) ion and arginine by using the composite film of electroinactive polypyrrole and polyion complex. / Komaba, Shinichi; Fujino, Yusuke; Matsuda, Tomoka; Osaka, Tetsuya; Satoh, Ikuo.

In: Sensors and Actuators, B: Chemical, Vol. B52, No. 1-2, 01.01.1998, p. 78-83.

Research output: Contribution to journalArticle

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